Enriching Mitochondria

The MitoSENS team has recently published a new protocol which enables researchers to isolate mitochondria from mammalian cells more efficiently and scalably than any prior technique, using only readily available and economical reagents and equipment.
Graphical Chemical Mito Prep_6_3_20 ANN

The MitoSENS team has recently published a new protocol which enables researchers to isolate mitochondria from mammalian cells more efficiently and scalably than any prior technique, using only readily available and economical reagents and equipment.

The full text is available to read for free via MethodsX: Rapid enrichment of mitochondria from mammalian cell cultures using digitonin

Abstract

We describe here a simple method to enrich mitochondrial fractions from mammalian cells for downstream analyses in the lab. Mitochondria purification involves cell lysis followed by separation of the organelles from the rest of the cellular components. Here, we use detergent to rupture the cell membrane of mammalian cells followed by differential centrifugation to enrich the organelles. Optimum conditions with respect to detergent concentration, time, sample size, and yield are discussed. The method’s utility in downstream analyses and ease of processing multiple samples simultaneously is also described. All the reagents in this method can be assembled in-house, are economical, and are comparable, if not superior, to commercially available kits in terms of mitochondrial yield and integrity.

  • Rapid enrichment of mitochondria from mammalian cells using commonly available reagents.
  • Multiple samples can be processed simultaneously.
  • Works over a wide range of sample size (1 million to 100 million cells).

Graphical Abstract

Share on facebook
Share on twitter
Share on reddit
Share on linkedin
Share on google
Share on email

Use of this Web site constitutes acceptance of the Terms of Use and Privacy Policy.

© 2021 SENS Research Foundation – ALL RIGHTS RESERVED

Thank you for Subscribing to the SENS Research Foundation Newsletter.

You can also

or

You can