SENS PubMed Publication Search
TFEB-dependent lysosome biogenesis is required for senescence
Rachel Curnock 1, Katy Yalci 1, Johan Palmfeldt 2, Marja Jäättelä 3 4, Bin Liu 3, Bernadette Carroll 1
...We find here that lysosomes in senescent cells are dysfunctional; they have higher pH, increased evidence of membrane damage and reduced proteolytic capacity. The significant increase in lysosomal content is however sufficient to maintain degradative capacity of the cell to a level comparable to proliferating control cells. We demonstrate that increased nuclear TFEB/TFE3 supports lysosome biogenesis, is a hallmark of multiple forms of senescence and is required for senescent cell survival. TFEB/TFE3 are hypo-phosphorylated and show constitutive nuclear localisation in senescence. Evidence suggests that several pathways may contribute to TFEB/TFE3 dysregulation in senescence.