SENS PubMed Publication Search
Horizontal mtDNA transfer between cells is common during mouse development
Nuria Marti Gutierrez 1, Aleksei Mikhalchenko 1, Hong Ma 1, Amy Koski 1, Ying Li 1, Crystal Van Dyken 1, Rebecca Tippner-Hedges 1, David Yoon 1, Dan Liang 1, Tomonari Hayama 1, David Battaglia 1, Eunju Kang 2, Yeonmi Lee 2, Anthony Paul Barnes 3, Paula Amato 1 4, Shoukhrat Mitalipov 1
...Here, we demonstrate the occurrence and extent of horizontal mitochondrial (mt)DNA acquisition between cells that are not in a parent-offspring relationship. Extensive single-cell sequencing from various tissues and organs of adult chimeric mice composed of cells carrying distinct mtDNA haplotypes showed that a substantial fraction of individual cardiomyocytes, neurons, glia, intestinal, and spleen cells captured donor mtDNA at high levels. In addition, chimeras composed of cells with wild-type and mutant mtDNA exhibited increased trafficking of wild-type mtDNA to mutant cells, suggesting that horizontal mtDNA transfer may be a compensatory mechanism to restore compromised mitochondrial function...