R. Rastmanesh, F. Marotta, M.K. Kantah, R. Nagpal, A. Lorenzetti, H. Takadanohara, H. Hashizume, C. Tomella, R. Kobayashi, D.H. Chui

The aim of the present study was to ascertain whether a poli-mix functional food VCC1-99 (a mixture of 42 extracts of non-GMO fruits and vegetables, one ml containing 400mg carbohydrates, 0.04mg protein, 0.04mg fat, 8mg vitamin C, 1.2mg vitamin E, 0.5mg coenzyme Q10, 6mg royal jelly, 5mg L-carnitine, Vibracell, Named srl, Lesmo, Italy) could affect hepatic adenosine triphosphate (ATP) level in mice aging from 1 to 24 months. Hundreds-twenty healthy Balb-C mice raised in a controlled vivarium were divided into 3 separate age-groups (40 mice each): young (one month), middle-age (12 months) and aged (24 months). All mice were given water ad libitum and standard chow. Each age-group was further split into 2 different treatment schedule: a) 1ml of VCC1-99 into drinking water or b) 1ml of a solution containing similar quantities of vitamin C, E and coenzyme Q10 as an antioxidant control (AC). Further 5 mice gor each age-group served as untreated control (UC). Animals were supplemented for 3 weeks. Steady-state hepatic ATP was assessed by using 31P-NMR spectroscopy before treatment and at weekly intervals. Fasting mice were anaesthetized with 50mg/kg pentobarbital intraperitoneal injection and immobilized on a shielding belt embedded with paramagnetic beads to minimize signal interference from abdominal muscles. The stability of magnetic field was tailored by adjusting the shim coils according to the flee induction decay of liver water protons. A calibration of NMR peaks of metabolic phosphorus for each mouse against a reference standard was also carried out. Normal ventilation of fresh air (1.0L/min) through a face mask was also assured during the test. Out of the overall NMR spectrum, variables considered were: β-ATP/ref. peak, inorganic phosphorus (Pi)/ref peak and β-ATP/Pi. No liver pH (calculated as chemical shift difference of Pi and α-ATP) changes were recorded irrespective of the age and treatment. As compared to UC, AC mice showed a significant enhancement of β-ATP/ref. peak ratio only in aged mice starting from 2nd week of treatment (+ 9.3-9.6% p

Keywords (Optional): 
functional food
liver steady state energetic status