Inorganic-organic hybrid scaffold composites are being developed with the aim of increasing the mechanical scaffold stability and improving their tissue interactions in cell culture.we have prepared the electrospun PCL fibrous webs incorporating amphiphilic PEG-POSS telechelic were prepared via electrospinning. The unique microstructure, morphology, thermal stability and wettability of the resulting PCL/PEG-POSS electrospun nanowebs were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and contact angle measurement, respectively. The addition of PEG-POSS telechelic strongly affected the fiber diameters and microstructures as well as the wettability, compared to pure PCL and PCL/PEG8.0k electrospun fibrous webs. The potential biomedical applications of such nanospun webs as a scaffolding material were also evaluated in vitro using mouse osteoblast-like MC3T3-E1 cells. The cell adhesion, spreading, and interaction behavior of pure PCL, PCL/PEG-POSS and PCL/PEG nanofibers were explored. It was found that the PEG-POSS telechelics containing electrospun PCL fibrous webs showed higher initial cell attachment than PCL/PEG8.0k due to the higher surface free energy of POSS siloxanes. The prepared PCL/PEG-POSS fibrous scaffolds were found to be nontoxic and to maintain the good adhesion ratio between cells and surface (about ~93 %) after cell culturing for 24 hrs.
polyhedral oligosilsesquioxane (POSS)