Nanog has been discovered that is essential for mouse and human embryonic stem cells (ESC) pluripotency and self-renewal. It also expressed in several adult murine tissues by using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. However, human Nanog transcripts have been isolated from adult bone morrow (EST, BF893620). Here, we study the Nanog gene expression profiling in the isolated mouse renal papillary cells that were confirmed by assessment of expression by Northern blots, RT-PCR. Mice renal cells whole RNA was got from fresh renal tissues, Phosphate Buffered Saline (PBS) infusion renal tissues, and the isolated mouse renal papillary cells respectively, as well as the renal papillary tissue from 18.5 days post-coitum (d.p.c.) fetal, 1-2 week young, 1-8 month adult and 24 month aging. Our analysis shows that, a very low expression level were detect in mouse renal tissues, and the renal papillary cells express more than other tissues with northern blot and RT-PCR. This data suggest that Kidney has its own Nanog expression cells exclude that from bone marrow derived cells, and Nanog expression loss in an age-dependent manner in the kidney, either due to developmental factors or aging, particularly in renal papillary tissue.