Authors: 
L. Danielyan, R. Schäfer, A. von Ameln-Mayerhofer, S. Verleysdonk, T. Klopfer, J. Geisler, M. Buadze, B. Proksch, G.H. Buniatian, C.H. Gleiter, W.H. Frey II
Category: 
Invited
Conference: 
Abstract: 

Organiser's Note: The presenter of this talk withheld their permission for video to be published.


Cell-based therapy is one of the future therapeutic strategies for neurodegenerative diseases. A big challenge is the method of cell delivery which may influence the graft survival, sufficient enrichment of therapeutic cells in the brain and avoidance of their distribution throughout the peripheral organs. We hypothesized that eukaryotic cells may bypass the blood-brain-barrier by migrating from the nasal mucosa into the brain. This hypothesis was tested by intranasal (IN) application of mesenchymal stem cells and glioma cells in young and adult mice and rats. Both cell types reached the olfactory bulb (OB) after IN-application and were found in other areas of the brain (cortex, hippocampus, striatum and cerebellum). After cells crossed the cribriform plate, two migration routes were observed: 1) migration through the OB to the hind parts of the brain; 2) entry into the CSF followed by entrance into the parenchyma from the cortex. Cell delivery to the brain was enhanced by pre-treatment of the nasal mucosa with hyaluronidase. This study shows a novel method of cell delivery to the brain using non-invasive IN delivery.

Keywords (Optional): 
intranasal
non-invasive
delivery
stem cells
CNS