Authors: 
T.B.L. Kirkwood
Category: 
Invited
Conference: 
Abstract: 

We have shown that important functional changes occur with ageing in the stem cells of murine intestinal epithelium, resulting in increased susceptibility to damage-induced apoptosis, impaired regenerative capacity, and altered DNA damage responses. More recently, in studies of human colonic epithelium we have described a surprisingly high burden of mtDNA mutation that increases strongly with age and appears to originate within the stem cells. Our results raise a number of important questions about the role played by mitochondrial mutation in stem cell ageing within the gut, and perhaps within other tissues. These detailed quantitative data on the frequency and distribution of individual mtDNA mutations can be used to address fundamental questions about the dynamics of their accumulation within proliferating tissue.

Keywords (Optional): 
Ageing
Stem Cells
Intestinal Epithelium
Mitochondrial Mutation