S. Mohammadzadeh, A.V. Kolesnikov, L.Yu. Prokhorov

Purpose: The probability of oncological diseases occurrence increases with the ageing. Avercectin C, antiparazitic preparation, has been shown recently to posses some anticancer properties. The purpose of work was to find out influence of avercectin C on growth of the transformed Chinese Hamster cells.

Materials and methods: The transformed Chinese hamster cells, line diiFAF28, 237 clone, grown in 24-well plate in the Iggle medium with 10 % of cattle serum without change medium and subcultivation. After 1 day growth of culture it was entered a preparation in various concentrations (0; 0.1; 0.25; 1.0; 5.0; 10.0; 15.0; 20.0 mkg / ml, 3 wells on 1 concentration) and estimated the growth rate of cells, saturation density and time of a life of cell cultures.

Results: Within the first 3 day growth rate of cells with preparations does not differ from growth rate of control culture. The growth rate of cells exposed in high concentration of aversectin significantly decreased in the 5-th day in comparison to the control and exposed in lover concentration. The cells exposed in 0.1 up to 5 mkg / ml of preparation reach the maximal saturation density (210 thousand cells on sq. cm and in the control over 499 thousand cells on sq. cm) on the 7-th day of cultivation, and then started to die. Cells with the high concentration still continue to grow up to 10-th day, but maximal cell density does not exceed 172 thousand cells on sq. cm. After 10 days of cultivation these cells start to perish. All cultures with aversectin perish on 10-12 days, but in the control the cells remain alive after 26 days of cultivation.

Conclusion: The preparation suppresses growth of the transformed cells. This suppression has cumulative character since after preparation introduction the cells continue to grow, and perish only after several cycles of division (approximately after 4 ones).

Keywords (Optional): 
Life span
Cell culture