F. Marotta, A. Kumari, H. Yadav, A. Polimeni, V. Soresi, A. Lorenzetti, Y. Naito, S. Jain

Ultraviolet (UV) radiation is one of the causative factors of DNA damage and inflammatory responses, and induces various skin lesions being a well-established epidemiological risk factor for photoageing and photocarcinogenesis. Moreover, the matrix metalloproteinases (MMP) production and subsequent ECM alteration are observed in photo-aged skin. Thus, it has been ascertained that UV triggers a cascade of signaling mechanisms which then cause a decrease of collagen and an increase of MMP, inflammation, epidermal DNA damage and apoptosis. Recently, a number of marine compounds have gained popularity for their cyto-protective and membrane-stabilizing effects. Thus, the aim of this study was to test the activity of a marine nutraceutical CH-1222 (containing DNA, collagen elastin and protein extracts together with grape skin extract, coenzyme Q10, lutein and selenium, Celergen®, R D Pharma, Switzerland) to exert a significant protection against UVA-induced photo-aging of human fibroblasts. Full-thickness human skin biopsies were obtained from sun-protected buttock skin of 22-39 years old healthy donors by punch biopsy. Cytotoxicity assay in a monolayer culture were checked before proceeding with the study and CH-1222 was found devoid of any cell damaging effect at any dosage in the medium. The cells were then pretreated with the CH-1222 for 24 h before UV irradiation as compared with a medium containing same quantity of coenzyme Q10, lutein and selenium as control (B-treatment). After pretreatment, cells were washed with PBS and then irradiated with UV in PBS. Zymography was carried out to detect the activity of secreted MMP-1 in culture supernatants, by using 10% polyacrylamide gels, electrophoresis and the bands were scanned by an image-analysis system and analyzed with an image software. Elastase activity inhibition was tested by measuring the amount of released p-nitroaniline and it was read by spectrophotometry. Assay of MMP-1 gene expression by RT-PCR was used as well. As compared to untreated control, UVA-irradiated samples exhibited a significant increase of secreted MMP-1 (115ng/ml vs 22ng/ml, p60%, p54%) elastase activity inhibition (p

Keywords (Optional): 
skin photoaging
marine extract
elastase activity inhibitor