F. Marotta, A. Polimeni, U. Solimene, A. Lorenzetti, E. Minelli, S. Jain, R. Rastmanesh, S. Sedriep, V. Soresi.

Alterations in collagen have been shown to be a cause of the clinical skin changes taking place during natural aging and increased elevated MMP expression and a concomitant reduction in collagen synthesis are common mechanisms of natural skin aging. Very recently it has been found that a significant decrease in mitochondrial membrane potential in samples from aged donors, accompanied by a significant increase in ROS levels. The aim of the present study was to test the activity of LD-1227 (containing high-purity caviar-derived DNA, collagen elastin and protein extracts from beluga sturgeon added with coenzyme Q10 and selenium, CaviarLieri®, Laboratoires Dom, Switzerland) in aged human skin in vitro and its potential beneficial role on aged skin mitochondria function. Human dermal fibroblasts were obtained under informed consent from the foreskin of healthy donors over 70 years old selected under proper strict criteria excluding main diseases. The cultures obtained were tested for the presence of mycoplasma using a PCR assay and only mycoplasma negative cultures were used. LD-1227 was found devoid of any cell damaging effect at any dosage in the medium by cytotoxicity assay in a monolayer culture were checked before proceeding with the study. The fibroblasts were isolated and grown to be then treated with or without LD-1227 for 72 h before analysis. The number and viability of released cells was quantified and the assay was repeated three times. Type I and III collagen and fibronectin levels in the conditioned culture media of human skin fibroblast cultures were measured. To test the effect of LD-1227 on fibroblast collagen mRNA and fibronectin gene expression, RNA isolation and reverse transcription polymerase chain reaction (RT-PCR) was employed. Moreover, relative level of cellular cytosolic ATP was assayed and reported as percent luminescence in relation to the luminescence of untreated normal cells while mitochondrial membrane potential (ΔΨm) were measured by fluorescence as well. Separate studies had shown that coenzyme Q10 and selenium had no effect on any of the above parameters tested except a trend improvement of cytosolic ATP but at high dosages (20 times higher than the ones contained into LD-1227). As compared to baseline, LD-1227 brought about a robust (>67%) collagen type I synthesis (p

Keywords (Optional): 
caviar-derived homogenate
chronological skin aging
collagen synthesis
mitochondria activity