F. Marotta, A. Kumari, R. Catanzaro, C. Tomella, U. Solimene, A. Lorenzetti, S. Jain, E. Minelli

The prevalence of metabolic syndrome is increasing worldwide in both developing and developed countries. Metabolic syndrome (MS) encompasses a clustering of risk factors for cardiovascular disease, including obesity, insulin resistance and dyslipidemia and have causative roles in the development of chronic kidney disease (CKD). On its turn, CKD leads to end-stage renal disease, cardiovascular disease and death. The relationship between CKD and metabolic syndrome may provide clues to better understand the role of lifestyle-related factors and the age-related decline in GFR. The aim of this study was to evaluate the influence of a DTS-phytocompound that we have recently shown to be protective in diabetic nephropathy (DTS: panax pseudoginseng, eucommia ulmoides, Kyotsu Jigyo, Tokyo, Japan) administration on oxidant-antioxidant balance, protein damage and lipid levels in kidney of rats administered high dose of fructose. Adult male Wistar rats were divided into four groups of 10 rats each. Groups A and D animals received starch-based control diet, while groups B and C animals were fed a high-fructose diet (60 g/100 g). Groups C and D rats additionally received DTS (50mg/kg/day) for 60 days. The extent of lipid peroxidation, enzymatic and non-enzymatic antioxidants and lipid levels were measured after 60 days. The accumulation of nitrated and oxidatively modified proteins in kidney was assessed by immunohistochemical study. Fructose-fed rats showed significantly higher levels of peroxidation end products, diminished antioxidant status, increased staining for the presence of 4-hydroxy-2-nonenal, 2,4-dinitrophenol and 3-nitrotyrosine protein adducts and lipid accumulation in kidney. DTS administration significantly decreased such renal alterations as well as mineral status abnormalities (partial normalization of higher Zn and lower Cu, Mg and Mn levels in liver and kidney of untreated MS-rats). The benefits of DTS in this model suggest the therapeutic use of DTS to counter the kidney changes associated with metabolic syndrome. Gender-related analysis of this interventional model is under way.

Keywords (Optional): 
metabolic syndrome
renal disease