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Abstract Archive

This searchable list includes the abstracts of all presentations given at a conference organised as part of the SENS series. We regret that the videos recorded at SENS3 and SENS4 are currently unavailable.

From disease-oriented to aging/longevity-oriented studies

Authors: A. Budovsky, K. Muradian, V. Fraifeld

Aging should be considered a major risk factor for life-threatening degenerative pathologies including atherosclerosis, cancer, neurodegenerative diseases, diabetes type II, osteoporosis, and sarcopenia. Although an apparent paradox, it appears that the most effective way to delay or even to avert the age-related pathology is to live longer. This is definitely exemplified by the phenomena of negligible senescence observed in certain species, exceptional longevity in humans, and by different experimental models of extended life span.

Keywords: Aging, diseases, epigenetics, lifespan extension,

Mitochondrial genome anatomy and species-specific lifespan

Authors: G. Lehmann, A. Budovsky, K. Muradian, V. Fraifeld

Several lines of evidence implicate mtDNA in the control of aging and longevity. Yet, there is a limited number of studies attempting to compare the basic mtDNA characteristics with species-specific (maximum) lifespan (MLS). Therefore, we examined correlative links between mtDNA composition and MLS of multicellular eukaryotes. For this purpose, we built a new database containing 144 species from 11 classes.

Keywords: mtDNA, maximum lifespan , , ,

Immune Regulation in Frail Elderly

Authors: G. Colonna-Romano, M. Bulati, S. Buffa, M. Pellicanò, G. Candore, C. Caruso

Frailty is a state of critically impaired homeostasis that results in heightened vulnerability to stressors. It is common in older persons and associated with adverse health outcomes. We have focused our attention on two different frailty systems, the first group of subjects affected by cardiovascular disease and the second one by Alzheimer's Disease. It is believed that cells with regulatory functions play a central role in the control of autoimmunity and inflammation.

Keywords: Regulatory cells, frailty, aging, ,

Regulatory T cells in frail elderly

Authors: M. Bulati, G. Colonna-Romano, A. Aquino, S. Vitello, C. Ventura, D. Lio, G. Candore, C. Caruso

Age-related diseases involve a wide range of factors such as chronic antigenic stress, inflammatory status and genetic background, which affect immune system and its homeostatic mechanisms. In the last years several types of cells involved in the regulation of the immune system have been identified. It is believed that cells with regulatory functions play a central role in the control of autoimmunity and inflammation.

Keywords: T regulatory, elderly, frailty, ,

Distinctive profile of pro-inflammatory receptors in naive and memory B cells of young, healthy elderly and Alzheimer Disease patients.

Authors: Bulati M, Buffa S, Martorana A, Gervasi F, Camarda C, Azzarello DM, Candore G, Lio D, Caruso C, Colonna-Romano G

It has been extensively demonstrated the impairment of humoral immune response in elderly humans, indeed they are characterized by a reduced ability to respond against new antigens and vaccines, due to a decrease both in percentage and absolute number of total B lymphocytes and modifications in the naïve/memory B cell compartments. Moreover, it is also well known that elderly are characterized by an “inflamed” environment (inflamm-aging).

Keywords: B lymphocytes, chemokine’s receptors, inflamm-ageing, Alzheimer Disease, inhibitory receptors

Memory B-cell subpopulations in aged

Authors: G. Colonna-Romano, M. Bulati, A. Aquino, S. Vitello, G. Clesi, C. Caruso

The literature on immunosenescence has focused mainly on the roles of T cells in the induction of the immune response and in the generation of the immunological memory. On the other hand, little is known about the B cell memory. According to the expression of sIgD and CD27 as markers for B memory cells (1), we have studied nave and memory B lymphocytes in our aged population. Our data show a decrease of nave IgD+CD27- B cells and a slight (not statistically significant) increase of memory IgD-CD27+ B cells.

Keywords: Ageing, CD27, IgD, B memory ,

The possibilities of use of "GRINIZATION" multinutrient functional peptide complex to prevent ageing

Authors: A.V. Bulavka, N.I. Linnik, O.G. Boyko

According to modern conception maximum life span is defined by the speed and particular qualities of organism metabolism. Metabolic disturbances cause accumulation of mutation, apoptosis, shortening of telomeres, etc. on the cell level, and on the organism level they cause ageing. The use of "GRINIZATION" multinutrient functional peptide complex, which contains digestible valuable proteins, fatty acids, vitamins and minerals in recommended quantities, ensures correction of metabolic disturbances and as a result prevents organism ageing effectively.

Keywords: GRINIZATION, aging, malignant neoplasms, hepatitis,

International Scientific Center for Research of the Future (Protection of Life)

Authors: V.V. Burdyuzha

Scientists worry state of our planet or more exactly they worry threats of life on the Earth. Global cataclysms took place in the past but then technological development did not allow to prevent these catastrophes. Other situation on our planet occurs now. Probably we can prevent local threats but we depend on the Sun in forward alternatively. Local threats are ecological, climatic, geodesic ones. The first and main threat is global warming in the result that greenhouse effect may develop.

Keywords: , , , ,

Evaluation of the specificity of hTERT gene induction by C0057684

Authors: Brown LK, Tersteege L, Burke P, Piatyszek MA, Andrews WH, Briggs LA, Wheeler J, Foster CA.

The inability to maintain telomere length in normal human cells lacking human telomerase (hTERT) activity results in cellular aging or replicative senescence. The identification of small molecules that induce telomerase activity in these normal cells may be useful for basic research aimed at studying telomerase regulation. Additionally, molecules found to induce hTERT expression, with high target selectivity, can possibly be used in therapeutic applications directed to alleviate or delay symptoms of aging. A high throughput screening (HTS) effort by Sierra Sciences L.L.C.

Poly(ADP-ribosyl)ation capacity of human T lymphocytes in vitro and in vivo as a function of age

Authors: Kathryn Annett [1], Alexander Bürkle [2], Muriel Malaisé [2], Orla Duggan [1], Robin Freeburn [1], Paul Hyland [3], Christopher Barnett [3], Anders Wikby [4], Graham Pawelec [5], Yvonne Barnett [1,3]
Audio: (Audio)

Poly(ADP-ribosyl)ation is an immediate response to DNA damage. This post translational modification of nuclear proteins is mostly catalysed by poly(ADP-ribose) polymerase-1 (PARP-1), and has been implicated in several crucial cellular processes including DNA repair, recombination and genomic stability.

Keywords: ageing, immunosenescence, T cells, poly(ADP-ribosyl)ation, PARP-1

Zinc, copper and amyloid in Alzheimer's disease

Authors: A.I. Bush

Background: The generation, aggregation, redox activity and toxicity of amyloid beta (Abeta) are modulated by neocortical transition metals (especially copper and zinc) in Alzheimer's disease (AD). Clioquinol is an 8-hydroxyquinoline that promotes matrix metalloproteinase clearance of Abeta, inhibits metal-mediated redox activity and oligomerization of Abeta, mitigating Abeta toxicity in vitro and brain Abeta accumulation in transgenic mice. Oral clioquinol also slowed cognitive decline in patients with moderately severe AD in a pilot phase 2 clinical trial.

Keywords: amyloid, Alzheimer's disease, copper, zinc, oxidation

Identification of biomarkers of human muscle aging and senescence

Authors: G. Butler-Browne, M.-C. LeBihan, A. Bigot, D. Furling, F. Svinartchouk, D. Bechet, V. Mouly

Muscle loss is the most common phenomenon of normal healthy aging and frequently leads to frailty and loss of independance in the elderly . It is important to understand the basic cellular mechanisms underlying this impairment. Decrease in muscle strength is associated with a decrease in cross sectional area of the muscle fibres, a decrease in capillary bed density as well as an increase in fibrotic tissue. A proteomic analysis is currently being carrried on biopsies of human skeletal muscle from young and old individuals in order to identify biomarkers of normal aging.

Keywords: , , , ,

Hypothalamic control of aging and longevity

Authors: Cai D.

The hypothalamus is a converging point that integrates metabolic, neural, neuroendocrine, and neuroimmune signals to affect the whole body physiology. The long-standing research interest of my research is to investigate the role of neural dysregulations, in particular in terms of neural inflammation in the development of aging and aging-related diseases. Our recent observations demonstrated that the hypothalamus contains adult neural stem cells, and IKK/NF-kB activation affects the fate of these cells and cause disease consequences in relation with aging and metabolic syndrome.

The Human Memome Project: Text-data analytics to find socio-cultural predictors of longevity utilising the quantified self, crowd sourcing and citizen science communities

Authors: Calimport SRG, Bentley B

Socio-cultural markers such as attitudes, behaviours, ideas, aspirations and interests were collected and correlated to previously identified social, psychological and quantitative predictors of lifespan.  Attitudes to whether a person wanted to live for as long as possible and if so in what condition were also surveyed.

 

Keywords: text, data science, markers, quantified self, citizen science

Adding beneficial genes to the body with phage integrases

Authors: M.P. Calos, A. Keravala, W.E. Jung, C.L. Chavez, L.E. Woodard, J.J. Hoyt, A. Farruggio, V. Gabrovsky

Gene therapy represents a creative strategy to correct or enhance gene products that improve health and longevity. We now possess a greatly improved understanding of the human genome. This information provides many therapeutic opportunities, but new tools are needed to manipulate the genome in safe and precise ways. Over the past several years, my lab has developed a novel strategy for adding genes to mammalian genomes by using a phage integrase, derived from the phiC31 phage of Streptomyces soil bacteria.

Keywords: gene therapy, hemophilia, muscular dystrophy, integration, phage integrase

Selective vulnerability in ageing rat sympathetic neurons

Authors: K.P. Gatzinsky, C. Thrasivoulou, M. Campioni-Noack, C. Underwood, T. Cowen

We have examined the hypothesis that differences in NGF uptake and transport determine vulnerability to age-related neurodegeneration. Neurons projecting to cerebral blood vessels (CV) have been found to be more vulnerable to this kind of degeneration than those projecting to the iris. Uptake of NGF was therefore examined in sympathetic neurons projecting from the superior cervical ganglion (SCG) to CV and iris in young and old rats by treating the peripheral processes of these neurons with different doses of I125-NGF.

Keywords: neurodegeneration, SCG, iris, cerebral blood vessels, NGF

New tricks for dealing with old cells?

Authors: Campisi J.

Cellular senescence is a crucial tumor suppressive mechanism that prevents the proliferation of cells at risk for neoplastic transformation. Numerous potentially oncogenic stimuli can induce a senescence response, which causes cells to enter a stable and essentially irreversible growth arrested state. Senescent cells are metabolically active and secrete myriad inflammatory cytokines, growth factors and other molecules that can alter the local tissue microenvironment. Senescent cells have been shown to accumulate with age and at sites of age-related pathologies.

Keywords: Cancer, DNA damage signaling, Inflammation, Matrix metalloproteinases

Cellular senescence, cancer and aging

Authors: J. Campisi
Audio: (Audio) (Slides)

Normal cells respond to DNA damage, dysfunctional telomeres and other potentially oncogenic events by entering an essentially irreversible state of arrested growth and altered function termed senescence. The senescence response, like the apoptotic response, is an important tumor suppressive mechanism for mammals. In addition, there is increasing evidence that the senescence response may be an example of evolutionary antagonistic pleiotropy and contribute to aging.

Keywords: , , , ,

Consequences of cellular senescence and prospects for reversal

Authors: J. Campisi
Audio: (Audio) (Slides)

Normal cells can respond to DNA damage, telomere dysfunction, and other potentially oncogenic events by entering an essentially irreversible state of arrested growth and altered function termed cellular senescence. Multiple lines of evidence indicate that the senescence response is an important mechanism for preventing the development of cancer among mammals. However, there is also evidence that cellular senescence may be an example of evolutionary antagonistic pleiotropy.

Keywords: tumor suppression, antagonistic pleiotropy, cellular senescence, cancer, aging

Association between PECAM-1 polymorphisms and atherosclerosis: results of a study on patients from Northern Italy

Authors: F. Listì, C. Caruso, C. Falcone, C. Boiocchi, M. Cuccia, G. Candore

Adhesion of circulating cells to the arterial surface is among the first detectable events in atherogenesis. Cellular adhesion molecules, expressed by the vascular endothelium and by circulating leucocytes, mediate cell recruitment and their transendothelial migration. Platelet endothelial cellular adhesion molecule-1 (PECAM-1), involved in this migration, has been associated with the development of atherosclerosis.

Keywords: CAD, PECAM-1, CD31, polymorphism, SNP

Immunosenescence and anti-immunosenescence therapies in ageing

Authors: G. Candore, S. Vasto, C.R. Balistreri, F. Listi, M.P. Grimaldi, G. Colonna-Romano, L. Scola, D. Lio, C. Caruso

Ageing is a post-maturational process that, due to a diminished homeostatic capacity and increased vulnerability, reduces responsiveness to environmental stimuli and is generally associated with an increased predisposition to illness and death. Accordingly, the incidence of infections, cancers, chronic inflammatory diseases such as atherosclerosis and Alzheimer's disease increases with age. These data suggest a key role for immunity in the survival of the elderly because susceptibility to these diseases depends at least in part on immune function.

Keywords: inflammation, immunosenescence, ageing, therapy,

Identification of three particular morphological phenotypes in sporadic thoracic aortic aneurysm (S-TAA): phenotype III as S-TAA biomarker in aged individuals

Authors: Balistreri CR, Pisano C, Di Maggio FM, Scola L, Maresi E, Lio D, Caruso C, Ruvolo G, Candore G.

Ageing has a striking impact on heart and vascular system, and particularly on the large elastic arteries, i.e. aorta, determining a multitude of changes at different structural and functional levels. As result, medial degeneration (MD) occurs, which can progress in aortic dissections and rupture. A characteristic example of MD is thoracic aortic aneurysm (TAA), potentially devastating and an important source of morbidity and mortality. It happens as an isolated manifestation or defined syndromes or familial forms.

Keywords: medial degeneration, S-TAA, , histopathological and immunohistochemical analyses, phenotype III as biomarker of S-TAA in aged individuals

Association between the HLA-A2 allele and Alzheimer disease

Authors: F. Listì, G. Candore, C.R. Balistreri, S. Vasto, M.P. Grimaldi, G. Colonna-Romano, A. Giacalone, V. Orlando, D. Lio, C. Franceschi, C. Caruso

The progressive neurodegenerative inflammatory age-related disease, Alzheimers disease (AD), is the most common cause of dementia in the elderly. Several factors, genetic and environmental, are involved in the onset of AD. Epidemiological data suggest that some genetic determinants of AD might reside in those polymorphisms for the immune system genes that regulate immune inflammatory responses, such as the Major Histocompatibility Complex (MHC). Therefore, MHC polymorphisms have been the focus of a large number of AD association studies.

Keywords: AD, HLA, immune response, ,

Nanoparticle-based artificial RNA silencing machinery for antiviral therapy

Authors: Cao YC.

RNA interference is a fundamental gene regulatory mechanism that is mediated by the RNA-induced silencing complex (RISC). Here we report that an artificial nanoparticle complex can effectively mimic the function of the cellular RISC machinery for inducing target RNA cleavage. Our results show that a specifically designed nanozyme for the treatment of hepatitis C virus (HCV) can actively cleave HCV RNA in a sequence specific manner.

Keywords: nanozyme, hepatitis C, RNA interference, antiviral, nanoparticle

Mouse Models and Human Aging

Authors: M.R. Capecchi
Audio: (Audio)

Gene targeting provides the means for creating strains of mice with designed alteration in any chosen genetic locus. This technology permits the evaluation of the functions of genes in the intact mammal and the systematic dissection of the most complex biological processes from embryogenesis to aging.

Keywords: mouse models of human aging , , , ,

Mr. Yuck meets the Grim Reaper: why living longer is not such a bad thing

Authors: A. Caplan
Audio: (Audio) (Slides)

Leon Kass, the Chair of President Bush's bioethics council maintains that efforts to extend human life are inherently immoral. He is not alone in this opinion. Daniel Callahan, Francis Fukuyama and Gilbert Meilander among others maintain that the drive to extend the human life span is wrong.

Keywords: finitude, ethics, life-extension, ,

Klotho polymorphisms and longevity: a systematic review

Authors: Di Bona D, Accardi G, Virruso C, Candore G, Caruso C

Nowadays is clearly evident that genetic background constitutes integral part of successful ageing and longevity. Many studies on long lived people have been conducted emphasizing the role of certain genes in long life. Classic case-control studies, genome wide association studies and high throughput sequencing have permitted to identify a variety of genetic variants seemingly associated to longevity. Over the years, ageing research has focused on insulin/IGF-1 signaling pathway because of its evolutionary conserved correlation with life-span extension in model animals.

Keywords: Klotho, Ageing, Systematic review, Longevity

Genetics of successful ageing: goals and future perspectives, a pharmacogenomics approach to prevent unsuccessful ageing

Authors: G. Candore, S. Vasto, C.R. Balistreri, F. Listi, M.P. Grimaldi, G. Colonna-Romano, L. Scola, D. Lio, C. Caruso

Several data indicate the presence of a strong familial component of longevity that is largely determined by genetics, and a number of possible associations between longevity and gene polymorphisms have been described. The study of centenarians who are the best example of successful ageing is a breakthrough strategy to get insight into the genetics of longevity. In this presentation we will discuss relevant data on longevity with particular focus on inflammation gene polymorphisms which could affect an individual's chance to reach the extreme limit of human life.

Keywords: longevity, genetics, inflammation, atherosclerosis, Alzheimer

Biology of longevity: role of the immune system

Authors: G. Colonna-Romano, G. Candore, A. Aquino, M. Bulati, S. Vitello, D. Lio, I.M. Rea, C. Caruso
Audio: (Audio) (Slides)

Ageing is an inexorable intrinsic process that affects all cells, tissues, organs and organisms. In fact, humans, as well as the other animals, are designed as a compromise to guarantee optimal survival until the time of reproduction based on natural selection that is effective until that age, so the post-reproductive physiology of an organism (i.e., ageing) is an epigenetic and pleiotropic manifestation of the optimisation for early fitness.

Keywords: Centenarians, Immunogenetics, Immune Response, Inflammation, Longevity

Mediterranean diet and longevity in Sicily: a survey in Sicani mountain population

Authors: C. Caruso, A. Marchese, C. Rizzo, S. Vasto

Since several years, increasing evidence suggests that the Mediterranean diet has a beneficial influences on several age-related diseases as metabolic syndrome and cardiovascular disease, hence showing protective effect on health and longevity. The effect of Mediterranean diet on human health has been reported in many population based studies and randomized trials, providing evidence for the positive effect on longevity of a dietary pattern rich in some nutritional food group.

Keywords: Centenarian, Longevity, Mediterranean diet, Olive oil,

Inflammation and oxidative stress in Alzheimer's disease: therapeutic implications

Authors: C. Caruso, C.R. Balistreri, G. Candore, G. Colonna-Romano, D. Di Bona, G. Duro, D. Lio, F. Listì, G. Scapagnini, S.Vasto

Alzheimer's disease (AD) is a heterogeneous and progressive neurodegenerative inflammatory disease which in Western society mainly accounts for clinical dementia. AD has been linked to inflammation and oxidative stress. Neuro-pathological hallmarks are senile plaques, resulting from the accumulation of several proteins and an inflammatory reaction around deposits of amyloid, a fibrillar protein, Abeta, product of cleavage of a much larger protein, the beta-amyloid precursor protein (APP) and neurofibrillary tangles.

Keywords: Alzheimer's disease, inflammation, pharmacogenomic, curcumin,

Looking for immunological risk genotypes

Authors: C. Caruso, A. Aquino, G. Candore, L. Scola, G. Colonna-Romano, D. Lio

Some people live in good health to great ages while others die relatively young, though we do not understand why this is so. However several studies show that longevity may be correlated with optimal functioning of the immune system. In fact, both longitudinal and cross-sectional studies performed in the last years have indicated that several functional markers of immune system may be used either as markers of successful ageing or conversely as markers of unsuccessful ageing.

Keywords: lymphocyte, IL-10, IFN-gamma, marker ,

Decreased numeric density of succinic dehydrogenase positive mitochondria in CA1 pyramidal neurons of 3xTg-AD mice

Authors: P. Fattoretti, M. Balietti, T. Casoli, B. Giorgetti, G. Di Stefano, C. Bertoni-Freddari, F. Lattanzio, S.L. Sensi

We employed the preferential copper ferrocyanide staining method to investigate neuronal mitochondrial metabolic competence in an animal model of Alzheimer's disease (AD). To that aim, we studied the appearance of signs of mitochondrial dysfunction in triple transgenic mice (3xTg-AD) that express mutant forms of the amyloid precursor protein (APP), presenilin-1 (PS1), and tau protein.

Keywords: energy metabolism, succinic dehydrogenase, Alzheimer's disease, mitochondria, 3xTg-AD

Effect of cognitive training on the expression of brain-derived-neurotrophic-factor (BDNF) in lymphocytes of mild cognitive impairment (MCI) patients

Authors: Casoli T, Giuli C, Balietti M, Giorgetti B, Solazzi M, Fattoretti P

Keywords: BDNF, MCI, cognitive training, qRT-PCR, lymphocyte

Vitamin E-deficiency and aging effect on expression levels of GAP-43 and MAP-2 in selected areas of the brain

Authors: T. Casoli, G. Di Stefano, A. Delfino, C. Bertoni-Freddari

The most widely accepted physiological function of vitamin E is its role as an important antioxidant in membranes, preventing oxidative damage to polyunsaturated lipids in the lipid bilayer. The brain is thought to be particularly vulnerable to oxidative stress due to its high rate of oxygen consumption along with its poor catalase activity and moderate amounts of superoxide dismutase and glutathione peroxidase. Moreover, it is known that the neurological abnormalities observed during aging are similar to those observed in vitamin E deficiency.

Keywords: MAP-2, GAP-43, aging, in situ hybridization, vitamin E-deficiency

Tissue-specific effect of age and caloric restriction diet on mitochondrial DNA content

Authors: P. Cassano, A.G. Sciancalepore, A.M.S. Lezza, C. Leeuwenburgh, P. Cantatore, M.N. Gadaleta

The decay of mitochondrial function is considered one among the major contributors of the age-related degenerative processes. Caloric restriction (CR) diet is widely known as the only treatment able to delay aging, increasing both the mean and maximum lifespan in a wide phylogenic range of animals including rodents and non human primates. Several evidences show that CR diet is able to prevent or reduce the age-dependent accumulation of oxidative damages to mitochondrial DNA (mtDNA) in different rodent tissues, mainly reducing the OH8dG content and the number of mtDNA deleted species.

Keywords: caloric restriction diet, aging, mitochondrial DNA content, Real Time PCR ,

Measurement of the 4834 bp mitochondrial DNA deletion level by Real Time PCR in aging rat liver subjected or not to caloric restriction diet

Authors: P. Cassano, A.G. Sciancalepore, A.M.S. Lezza, C. Leeuwenburgh, P. Cantatore, M.N. Gadaleta

Aging is a fascinating and highly important topic because of its great social relevance and scientific complexity. One of the most important theories to explain this multifactorial process is the free radical theory. Such theory involves the damaging role of reactive oxygen species (ROS) on mitochondrial molecular components as lipids, proteins and mitochondrial DNA (mtDNA).

Keywords: aging rat, Real Time PCR, caloric restriction, mtDNA deletion ,

Analysis of endothelial function by radiofrequency coupled with two-dimensional echocardiography: the value of flow-mediated vasodilation. A study of 25 cases

Authors: X. Castellon, V. Bogdanova

Aim of the study: Endothelial dysfunction is an early indicator of cardiovascular disease which precedes clinical manifestations. The primary objective of the present study was to perform early-stage screening of patients presenting cardiovascular risk factors with endothelial dysfunction in order to optimise care provision. This will reduce the short- and medium-term occurrence of cardiovascular diseases.

Keywords: endothelial dysfunction, cardiovscular disease, radiofrequency, flow-mediated vasodilation, reactive hyper

Protocol of screening of sub clinical atherosclerosis by radio frequency (RF) coupled with 2D echo.

Authors: X. Castellon, V. Bogdanova

The study was carried in Athis Mons France between 2008 and the early 2011 on a cohort of 350 patients with cardiovascular risk factors. Introduction: Atherosclerotic lesions increase with aging and the changes favored by risk factors (diabetes, obesity, hypertension, dyslipidemia, smoking) and hence the interest of making an early detection of sub clinical atherosclerosis. The study of intima media thickness and speed of the wave pulse based on age, can detect patients at risk with increased threshold values, which reflects early atherosclerotic impregnation of the arterial wall.

Keywords: Early detection, atherosclerosis, age, , QIMT

QIMT by radio frequency (examination of tracking). Method of evaluation thickness of the intima media among patients having cardio vasclaires risk factors

Authors: X. Castellon, V. Bogdanova, J.C. Charniot

Method: QIMT by Radio frequency.Method of the examination used is in conformity with the IMT protocol of Mannheim standarts of normal values according to age, measurements of IMT based on the radio frequency. This study has been done with 150 patients(100 men and 50 women),aged between 45 and 60 years,having cardiovascular risk factors dyslipidemy,standard diabetes II noncomplicated, hypertension and tobacco).All patients having atheroma plaque located in the carotids have been excluded from the study.

Keywords: QIMT: calculation thickness of intima media, RF: radio frequency, IMT: thickness intima media, VIF: vasodilatation,

Longer telomeres in mitochondrial lineages doubly-selected for extreme longevity and late female reproduction

Authors: Berrett MG, Cawthon RM, Kerber RA, O'Brien E.

Successful reproduction late in life in women is associated with familial longevity.  Therefore, some genetic factors may slow both overall aging and reproductive aging in females.  Here we investigate mitochondrial genetic contributions to longevity in both sexes and late reproduction in women.

Keywords: telomere, mtDNA, reproduction, aging, longevity

Oxidative stress implication in acute heart failure

Authors: J.C. Charniot, N. Vignat, D. Bonnefont Rousselot, V. Bogdanova, K. Zerhouni, J.J. Monsuez, J.Y. Artigou

Aim of the study: Oxidative stress implication (OS) is more important in pathology: ischemia reperfusion sequence (acute coronary syndromes, cardiac surgery, transplantation...). In heart failure, OS implication is less known. This study aimed at evaluating OS in acute heart failure.

Method: patients consecutively hospitalized in department of cardiology with a first oedema pulmonary complicated a dilated cardiomyopathy (CMD).

Keywords: oxidative stress, oxygen free radicals, acute heart failure, cardiomyopathy, arrhythmia

Excitotoxic neurodegeneration induced by intranasal administration of kainic acid in C57Bl/6 mice

Authors: Z. Chen, H.-G. Ljunggren, N. Bogdanovic, I. Nennesmo, B. Winblad, J. Zhu

Glutamate excitotoxicity plays a key role in inducing neuronal cell death in many neurological diseases, such as Alzheimer's disease. In mice, administration of kainic acid, an analogue of the excitotoxin glutamate, results in hippocampal cell death and seizures. Kainic-acid-induced seizures in mice provide a well-characterized model for studies of human neurodegenerative diseases. However, C57BL/6 mice, which are often used for genetic analyses and transgenic and knockout studies, are resistant to excitotoxicity induced by subcutaneous administration of kainic acid.

Keywords: kainic acid, excitotoxic, neurodegeneration, hippocampus, C57BL/6 strain

Rescuing Aged Mesenchymal Stem Cells by Exposure to a Young Extracellular Matrix

Authors: Y. Sun, W. Li, R. Chen, Z. Lu, J. Ling, Q. Ran, R.L. Jilka, X.-D. Chen

Previously, we reported that in both mouse and human models a native extracellular matrix (ECM) generated by bone marrow cells dramatically promoted mesenchymal stem cell (MSC) proliferation, preserved the stem cell properties, and enhanced their capacity for skeletogenesis (Chen et al, 2007, JBMR, 22:1943; Lai et al, 2010, Stem Cells Dev., 19:1095). This led us to investigate whether culturing aged MSCs on an ECM could improve their number and quality.

Keywords: Age , Mesenchymal Stem Cells, Extracellular Matrix, Reactive Oxygen Species,

Sirtuin regulation of metabolism and stem cells

Authors: Brown K, Qiu X, Xie S, Shin J, He M, Liu Y, Chen D.

The metabolic network is coordinately regulated in response to nutritional status to maintain homeostasis. Perturbed metabolic homeostasis is integral to the aging process and underlies many aging-associated diseases. Recent studies strongly suggest that metabolic enzymes are concertedly regulated via acetylation to allow coordination of the directionality and the rate of the metabolic flux upon changes in nutritional status. This mode of metabolic regulation is conserved evolutionarily and is regulated by the sirtuin family of deacetylase.

A high-throughput screening system for human genes extending life-span

Authors: C. Chen, R. Contreras

We developed a high-throughput functional genomic strategy that allows identification of human genes prolonging life-span in the baker's yeast Saccharomyces cerevisiae. The method is based on isolating yeast mother cells with extended number of cell divisions as indicated by the increased number of bud scars on their surface. Screening of a human HepG2 cDNA expression library in yeast resulted in the isolation of 12 yeast transformants with a potentially prolonged life-span. The transgene in one of the lines was identified as ferritin light chain (FTL) and studied in more detail.

Keywords: aging, lifespan, stress, yeast ,

Phage integrase for targeted gene therapy in mouse models

Authors: Qiu J, Zhang T, Zheng Q, Chen-Tsai RY

 

Generally biological approach to a problem of aging

Authors: V.E. Chernilevsky

Purpose: On a basis of the general biological approach to a problem of aging the prospects of life prolongation of the man are studied. Materials and methods:. The comparative analysis of aging of organisms of various systematic groups has been carried. Results: The aging is a decrease of viability organism owing to delay of self-renovation of cells, organs and tissues. The self-renovation - basic property alive, is provided at the expense of system stem cells (SC) during all life.

Keywords: Aging, Life span prolongation , , ,

Lowering of metabolism - a possible method of human life span prolongation

Authors: V.E. Chernilevsky

Purpose: The possibilities of lowering basal metabolism (LBM) in man with aim of longevity have been investigated. The method is based on the data that at many mammalia in state of hypobiosis BM is lowered very much and aging is delyed independently from the mechanisms, and after hypobiosis the process of self-renovation of tissues is activated, In addition the life span of some species (Microtus, Soricidae etc,) is able to increase in several times.

Keywords: Lowered metabolism, Longevity , , ,

Model of aging taking into account packing of cells

Authors: V.E. Chernilevsky, A.N. Zajarny

Purpose, material and methods: The numerical experiment was carried out on a model of organism for valuation mechanisms of aging. Results: The cells (C) were substituted for deformable spheres in limited volume. Proliferation simulated by the doubling of C with the given coordinates.

Keywords: Cellular packing, Aging, , ,

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