Abstract Archive

This searchable list includes the abstracts of all presentations given at a conference organised as part of the SENS series. We regret that the videos recorded at SENS3 and SENS4 are currently unavailable.

On the pro-inflammatory phenotype of senescent cells: the p53-mediated ICAM-1 expression

Authors: D. Kletsas, H. Pratsinis, G. Mariatos, V.G. Gorgoulis

Senescent cells are characterized by an pro-inflammatory phenotype, possibly leading to detrimental effects on tissue homeostasis. Microarray analysis has shown that one of the genes found to be over-expressed in cellular senescence is ICAM-1 (Intracellular Adhesion Molecule-1) a crucial receptor in the cell-to-cell interaction, a process central to the reaction to all forms of injury. Its expression is up-regulated in response to a variety of inflammatory mediators, mostly via the NF-kB signalling pathway.

Keywords: Senescence, inflammation, p53, ICAM-1 ,

Directing the Repair of Damaged DNA and Genetic Mutations in vivo

Authors: E.B. Kmiec
Audio: (Audio)

DNA mutations either inherited or acquired during the lifecycle of the cell can affect multiple metabolic pathways. DNA damaging agents accelerate the rate of mutation and in some cases are responsible for the development of serious disease states. While a number of potential therapies are being developed to treat such disorders, the more direct approach is one in which the mutated base is corrected at its chromosomal location. Reversing such mutations would have a serious impact on the disease and likely lead to a permanent correction of some inherited diseases.

Keywords: gene repair, oligonucleotides , , ,

Longevity, ionizing radiation, and hormesis: the reliability-theory point of view

Authors: V.K. Koltover

Despite the phenotypic variety, aging of all organisms is governed by the universal quantitative laws. First, each species is characterized by the species-specific maximal life-span potential. Second, the growth of mortality rate with age obeys to the statistics of extremes (usually, the Gompertz law of mortality, h(t) = ho exp(γt), where ho и γ are the time-independent parameters), that has been confirmed for people and for other species of mammals, flies, mollusks, etc.

Keywords: aging, longevity, reliabitiy, free radicals, hormesis

Apolipoprotein E genotype and the age at menopause

Authors: J. Koochmeshgi, S.M. Hosseini-Mazinani, S.M. Seifati, N. Hosein-Pur-Nobari, L. Teimoori-Toolabi

Apolipoprotein E plays an important role in lipid transport and metabolism. E2/E3/E4 polymorphism of apolipoprotein E (APOE) gene has been implicated in a number of age-associated diseases and conditions- e.g.: Alzheimers, coronary heart disease, and osteoporotic fractures- with APOE 4 allele being associated with these conditions. In an interesting parallel, the age at menopause in women shows a connection with the same age-associated diseases and conditions, an early age at menopause being a risk factor.

Keywords: Apolipoprotein E, Menopause, Life History , ,

An appetite for death

Authors: J. Koochmeshgi

Sustained restriction of food intake can extend life in a diverse array of organisms. This phenomenon has been demonstrated in species of rotifers, nematodes, arthropods, and studied in detail in rodents. Here, we propose a hypothesis as a framework for interpreting this phenomenon. Our hypothesis connects food intake, reproduction, health, and longevity. We draw attention to a feature of dietary restriction- a concomitant decline in reproductive capacity. This has been shown in several species.

Keywords: Dietary Restriction, Aging, , ,

Reproductive switch and aging- the case of leptin change in dietary restriction

Authors: J. Koochmeshgi

Dietary restriction experiments provide a model for exploring the phenomenon of aging. Plasma levels of several biomolecules are known to change as a result of dietary restriction and these biomolecules have been considered for their possible role in aging. We have proposed a hypothesis for interpreting extension of life by dietary restriction. It posits that normal food intake is geared toward optimizing the internal milieu for reproduction, even though some components of this milieu may be detrimental to health in the long term.

Keywords: Leptin, Dietary Restriction, , ,

Mechanisms for the Negative Effects of elevated Superoxide Dismutase Levels

Authors: A. Kowald

One of the most important antioxidant enzymes is superoxide dismutase (SOD), which catalyses the dismutation of superoxide radicals to hydrogen peroxide. The gene for CuZnSOD lies in humans on chromosome 21 and its activity is increased in patients with Down syndrome. But instead of being beneficial, increased lipid peroxidation is associated with this increased expression and also studies on bacteria and transgenic animals show that high levels of SOD actually lead to increased lipid peroxidation and hypersensitivity to oxidative stress.

Keywords: oxidative stress, superoxide, mathematical model , ,

The molecular chaperones and the process of cellular immortalization in vitro

Authors: J. Kroll

The suggestion of causative involvement of the molecular chaperones in the process of cellular immortalization in vitro is based on the following observations.

Keywords: Cell immortalization, Molecular chaperones , , ,

Evidence for increased oxidative stress during replicative senescence of human peritoneal mesothelial cells in vitro

Authors: K. Ksiazek, A. Breborowicz, A. Joerres, J. Witowski

Increased generation of reactive oxygen species (ROS), depletion of antioxidant factors (such as glutathione; GSH) and accumulation of oxidative modifications of vital molecules (exemplified by 8-hydroxyguanine; 8-OH-dG) have all been implicated in the development of senescent phenotype. We have therefore examined whether replicative senescence of human peritoneal mesothelial cells (HPMC) in vitro is associated with increased oxidative stress.

Keywords: oxidative stress, peritoneal mesothelial cells, replicative senescence , ,

Replicative senescence of human peritoneal mesothelial cells in vitro - a new model for cytogerontological studies

Authors: K. Ksiazek, A. Breborowicz, J. Witowski

In vitro models of ageing have been developed for several cell types including fibroblasts, endothelial cells, keratinocytes and lymphocytes. However, very little is known about senescence of human peritoneal mesothelial cells (HPMC) that form the largest cell population in the peritoneal cavity and play a crucial role in intraperitoneal homeostasis and, host defense.

Keywords: peritoneal mesothelial cells, replicative senescence , , ,

Relocalized redox-active lysosomal iron is an important mediator of oxidative stress-induced DNA damage

Authors: T. Kurz, A. Leake, T. von Zglinicki, U.T. Brunk

Hydrogen peroxide-induced oxidative damage to nuclear DNA is supposed to involve Fenton-type chemistry with redox-active iron producing highly reactive hydroxyl radicals. Hydroxyl radicals are very short-lived and therefore act mainly at the site of their origin. To induce DNA damage by hydroxyl radical attack redox-active iron would have to be located close to DNA. The presence of free iron in the nucleus has never been convincingly shown. Recently, lysosomes as sites of intracellular digestion (e.g. of metallo proteins) were suggested as a pool of redox-active iron.

Keywords: Desferrioxamine, DNA damage, lysosomes, oxidative stress, redox-active iron

Cost of reproduction and susceptibility to heat stress in fruitflies

Authors: S. Ladonni, S.M. Hosseini-Mazinani, J. Koochmeshgi

Studies in several species have revealed that changes in reproductive activity result in inverse changes in lifespan. It is interesting to know how this "cost of reproduction" is incurred. Earlier, investigators had shown that mated female fruitflies have significantly shorter lifespan than virgin females. Recently, it has been demonstrated that mated young female fruitflies are significantly more susceptible to oxidative stress and starvation than virgin ones.

Keywords: Cost of Reproduction, Fruitfly, Heat Stress , ,

Intervening on cardiovascular aging in health to beat cardiovascular disease

Authors: E.G. Lakatta
Audio: (Audio)

Age per se, confers the major risk for cardiovascular (CV) disease because specific pathophysiological mechanisms that underlie these diseases become superimposed on cardiac and vascular substrates that have been modified by an "aging process". In healthy humans, rigorously screened to exclude CV disease, non-human primates (NHP) and rodents, the large elastic arteries become dilated and stiffen and the intima thickens, and exhibits cell and sub-cellular features that resemble those that occur during vascular injury.

Keywords: aging, hearts, arteries, age-associated disease, interventions on aging of hearts and arteries

Cell signaling and apoptosis in aging

Authors: C. Leeuwenburgh
Audio: (Audio) (Slides)

There is strong evidence that mitochondrial dysfunction in vivo plays a significant part in the pathogenesis of neurological disorders and sarcopenia (atrophy and loss of skeletal muscle myofibers) (1-4). Life-long caloric restriction (CR) has been shown to have neuroprotective effects and prevents the age-associated loss in muscle fibers as well as function, but the mechanisms in vivo are poorly understood.

Keywords: apoptosis, aging, inhibitors, caspases, function

Medical Time Travel as a Bridge to Negligible Senescence

Authors: J.B. Lemler
Audio: (Audio)

If one accepts the feasibility of engineering negligible senescence, the benefits of the endeavor depend on when it succeeds. Many of those who would like to benefit from engineered negligible senescence will likely perish before it can be accomplished. There is, however, a potential "safety net" for such individuals, which can be called medical time travel. It is based on one fact and two assumptions. The fact is that at the boiling point of liquid nitrogen, changes in biological systems are generally agreed to be negligible for periods of hundreds to thousands of years.

Keywords: , , , ,

Glutathione metabolism during aging and in diseases

Authors: R.-M. Liu
Audio: (Audio)

The concentration of glutathione (GSH), the most abundant intracellular non-protein thiol and important antioxidant, declines with age and in some age-related diseases. The underlying mechanism, however, is not clear. The previous studies from this laboratory showed that the age-dependent decline in GSH content in Fisher 344 rats was associated with a down regulation of glutamate cysteine ligase (GCL), the rate-limiting enzyme in de novo GSH synthesis.

Keywords: glutathione, aging, GCL, estrogen, Alzheimer's disease

Association of MMPs, TIMPs and proteoglycans with aging and osteoarthritis in mouse temporomandibular joint

Authors: A. Gepstein, G. Arbel, E. Livne

The temporomandibular joint (TMJ) is an important growth and articulation center in the craniofacial complex. In aging it develops spontaneous degenerative osteoarthritic (OA) lesions. Metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (Timps) play key role in extracellular matrix (ECM) remodeling and degradation. Gelatinase activities and immunohistochemical localization of MMP-2, -3, -8, -9, -13, and TIMP-1, -2 were examined in mandibular condyle cartilage of neonatal mice till 18-months old.

Keywords: Aging, Osteoarthritis, Articular cartilage, MMPs, Proteoglycans

Neutrophil superoxide generation and phagocytosis are reduced in elderly trauma patients: relation to increased infections rates and altered corticosteroid levels

Authors: S.K. Butcher, H. Chahal, V. Killampali, E.K. Alpar, J.M. Lord

As humans age their ability to combat infections declines as a result of an age-related loss of immune status. We have shown that the ability of neutrophils to ingest E.coli is reduced in healthy elderly humans and here we report the effects of a common trauma, hip-fracture, on infection susceptibility and neutrophil function in elderly humans. 44 elderly patients (mean age 82.5 years) with a hip-fracture and 9 young subjects (mean age 25.9 years) with a single limb fracture were recruited to the study.

Keywords: Neutrophil, Trauma, Corticosteroids , ,

The role of cellular senescence may be to prevent division of neighboring cells

Authors: M.D. Lynch

It has long been suspected that cellular senescence is an anti-cancer mechanism however it has been difficult to understand the advantage for the organism of retaining mutant cells in a post-mitotic state rather than simply deleting them by apoptosis. It is proposed that in certain circumstances apoptosis promotes neoplasia by causing neighboring cells to divide and that the role of cellular senescence is to prevent this.

Keywords: replicative senescence, apoptosis, stem cells, cancer ,

Aging and vitamin E deficiency are responsible of altered RNA pathways

Authors: M. Malatesta, C. Bertoni-Freddari, P. Fattoretti, B. Baldelli, S. Fakan, G. Gazzanelli

Ribonucleoprotein (RNP) containing structural constituents in hepatocyte nuclei of adult, old and adult, vitamin E-deficient rats were investigated to assess the effect of aging and increased oxidative stress on nuclear functions.

Keywords: Vitamin E deficiency, oxidative stress, ribonucleoproteins, mRNA, immunocytochemistry

Cirrhosis progression as a model of accelerated senescence: affecting the biological aging clock by a breakthrough biophysical methodology

Authors: G. Marineo, F. Marotta, G. Sisti

Besides transplantation (OLTx), liver cirrhosis has no valid therapeutic options and a relentless progression with overcoming complication is still a dismal prospective while OLTx waiting lists hugely enlarge. Quite recently, it has been shown that healthy livers undergo and age-related functional decay. Thus, a pilot study was designed with a novel biophysical methodology as a new treatment modality. Such non-invasive technique has as a main principle the "decrease of entropy", which can be artificially obtained through an electromagnetic-driven delivery of "energy clusters".

Keywords: biophysics, entropy-variation, liver cirrhosis, regeneration,

The aging/precancerous gastric mucosa: a preliminary antioxidant interventional trial

Authors: F. Marotta, R. Barreto, H. Tajiri, H. Fuji, E. Fesce

The aim of this study was to test the effect of antioxidants supplementation on enzymatic abnormalities and free radicals-modified DNA adducts associated with pre-malignant changes in the upper gastrointestinal mucosa of elderly patients with longstanding, HP-negative chronic atrophic gastritis (CAG). Sixty patients (mean age: 75, 67-81) with known atrophic gastritis and intestinal metaplasia were selected.

Keywords: chronic atrophic gastritis, antioxidants, 8-OHdG, vitamin E, Immun-Age

SMP30 knockout (SMP30-KO) mouse as an ageing mode

Authors: N. Maruyama, A. Ishigami, M. Kuramoto, S. Handa, S. Kubo, Y. Kasahara

We established a mouse strain lacking Senescence Marker Protein-30 (SMP30). Its life span is shortened. With ageing the most organs of this strain show various phenomena such as lipid deposition, degeneration of mitochondria, prominent lysosomes, lipofuscin granules in comparison with wild type strain. This strain is more sensitive to apoptosis induction than wild type because of insufficiency of calcium pump activity in plasma membrane. Since SMP30 expression is modulated by oxidative stress, dietary restriction can enhance the amount of SMP30 in liver and kidney.

Keywords: SMP30, apoptosis, organ injury, animal model, oxidative stress

A Longitudinal Study of Skeletal Muscle Aging and The Impact of Dietary Restriction in Rhesus Monkeys

Authors: S.H. McKiernan, R.J. Colman, J.M. Aiken

Sarcopenia is the age-related loss of skeletal muscle mass and function. Dietary restriction (DR) is the only known intervention that reduces the effects of age on muscle mass loss; however, nearly all studies have been conducted on rodent species. The purpose of this study is to monitor the effects of DR on age-related changes in muscle mass and muscle fibers, in the same rhesus monkeys over time.

Keywords: rhesus monkey, dietary restriction, sarcopenia , ,

Mitochondrion-mediated apoptosis is enhanced in long-lived alphaMUPA transgenic mice and calorically restricted wild-type mice

Authors: O. Tirosh, I. Zusman, S. Yahav, R. Miskin

Caloric restriction (CR) can extend the life-span of multiple species and is the only intervention known to attenuate aging in mammals. Mechanisms mediating the CR influence are as yet unclear. To get insight into these mechanisms we took advantage of alphaMUPA transgenic mice that have previously been reported to spontaneously eat less and live longer compared with their wild-type (WT) control.

Keywords: caloric restriction, mitochondria, apoptosis, alphaMUPA transgenic mice, aging

Lack of correlation between mitochondrial ROS production and lifespan in Drosophila

Authors: S. Miwa, M.D. Brand

Reactive oxygen species (ROS) are thought to play a crucial role in determining longevity. ROS are produced by mitochondria and can attack and damage surrounding macromolecules. Accumulation of damage is suggested to be a major causal factor for the age-associated decline in physiological functions.

Keywords: mitochondria, ROS, drosophila, caloric restriction ,

Zinc, Immunomodulation, ageing and successful ageing: role of Metallothionein

Authors: E. Mocchegiani, R. Giacconi, M. Muzzioli, C. Cipriano
Audio: (Audio)

Extrathymic immune pasticity is pivotal for health ageing. Extrathymic T-cell subsets display age-different patterns during the circadian cycle. Immune remodelling occurs in young and very old age leading individuals as "high responders" to external noxae. Limited remodelling appears in old age. Therefore, old individuals are "low responders" with the appearance of age-related diseases. NKT and CD4+ cells alpha/beta and gamma/delta are pivotal because involved in polarising Th1/Th2 response. Different pattern of extra-thymic NKT cell exists during the circadian cycle.

Keywords: , , , ,

Influence of Avercectin C on Growth of the Transformed Chinese Hamster Cells

Authors: S. Mohammadzadeh, A.V. Kolesnikov, L.Yu. Prokhorov

Purpose: The probability of oncological diseases occurrence increases with the ageing. Avercectin C, antiparazitic preparation, has been shown recently to posses some anticancer properties. The purpose of work was to find out influence of avercectin C on growth of the transformed Chinese Hamster cells.

Keywords: Avercectin, Life span, Cell culture , ,

Research of the low doze gamma-irradiation influence on life span and aging speed of Drosophila melanogaster

Authors: A.A. Moskalev

Researches of radioinduced life span alteration of Drosophila which is carried out in our laboratory in 1996-2003 years, have revealed interrelation between mutations of several genes of DNA repair and apoptosis pathways with low doses ionizing irradiation and speed of aging.

Keywords: ageing, life span, Drosophila, apoptosis, radiation

Antibodies Against beta-Amyloid Slow Cognitive Decline in Patients with Alzheimer's disease

Authors: R.M. Nitsch, C. Hock
Audio: (Audio)

Immunization against beta-amyloid can reduce neuropathology and improve impaired behavior in transgenic mice. Whether antibodies against beta-amyloid are also effective in Alzheimer's disease (AD) is unknown. We have previously described the generation of antibodies against beta-amyloid in AD patients who received a prime and a booster immunization of aggregated Abeta42 in a placebo-controlled randomized trial.

Keywords: Vaccination, APP, dementia, amyloid, Abeta

Algae extract protection effect on oxidized proteins level in human keratinocytes

Authors: C. Nizard, S. Poggioli, C. Heusele, A.L. Bulteau, M. Moreau, A. Saunois, S. Schnebert, B. Friguet

Modification of proteins by reactive oxygen species is implicated in different disorders. The proteasome is a multicatalytic proteinase in charge of intracellular protein turnover and of oxidized proteins degradation. Consequently, proteasome function is very important in controlling the level of altered proteins in eukaryotic cells. Evidence for a decline in proteasome activity during skin photo-aging has been provided by us in Bulteau et al. (2002).

Keywords: proteasome, oxidized proteins, skin, ,

Heat Shock Protein (HSP) 47 expression in aged, photo-aged and senescent normal human fibroblasts. Modulation by salix alba extract

Authors: E. Noblesse, C. Boisde, M. Moreau, A.M. Faussat, S. Schnebert, C. Nizard

HSP 47 is a specific chaperone of pro-collagen. This heat shock protein is responsible for the correct 3-dimentional organization of pro-collagen and its control-quality prior secretion. The aim of the study is to evaluate the level of HSP47 in aged, photoaged and senescent fibroblasts and its modulation by a plant extract (Salix Alba).

Keywords: HSP47, fibroblast, collagen organization , ,

How an individual fecundity pattern looks in Drosophila and Medflies

Authors: V.N. Novoseltsev, R. Arking, J.R. Carey, J.A. Novoseltseva, A.I. Yashin

Reproduction is usually characterised by mean-population fecundity pattern. Such a pattern has a maximum at earlier ages and a gradual decline of egg production subsequently. We show that individual fecundity trajectory do not follow such a pattern. In particular, it has no maximum. The three-stage pattern, which includes maturation, maturity and reproductive senescence, is more appropriate description of individual fecundity. The mean-population maximum in fecundity rate arises as a by-product of averaging of "flat" individual fecundity patterns across the population.

Keywords: individual fecundity pattern, drosophila, medfly , ,

Obesity, Infectious Diseases, and Forecasts of Human Life Expectancy + The Demographic Impact of Immortality

Authors: S.J. Olshansky, D. Ludwig, B.A. Carnes, J. Brody, L. Hayflick, R. Butler
Audio: (Audio)

Recent forecasts of human life expectancy at birth made by mathematical demographers anticipate a steady rise in the 21st century to 100 years or more -- an anticipated extension of a historical trend that has lasted for more than 150 years. Such forecasts are based entirely on atheoretical and abiological assumptions while ignoring the biodemographic, biomechanical, biochemical, and stochastic constraints on the duration of life.

Keywords: life expectancy, mortality, forecasts, lifespan, immortality

The phenotypic expression of age-associated mtDNA deletion mutations in rat skeletal muscle is fiber type-dependent

Authors: J.W. Pak, J.M. Aiken

Mitochondrial DNA (mtDNA) deletion mutations can accumulate to levels that result in electron transport system (ETS) abnormal regions (cytochrome c oxidase negative, succinate dehydrogenase hyperreactive) in skeletal muscle fibers of aged rats. This ETS abnormal phenotype was primarily observed in type II fibers. MtDNA sequence analysis of microdissected portions of single fibers containing these ETS abnormal regions identified deletion mutational hotspots near cyt b and COX subunit genes in the major arc region of the mitochondrial genome.

Keywords: mitochondrial DNA, deletion mutations, ETS abnormality, fiber type,

Functional Efficiency of the Senescent Cells: Replace or Restore?

Authors: S.C. Park
Audio: (Audio)

It is generally accepted and even believed that aging is a phenomenon of irreversibility, inevitability and universality with parenchymal loss and functional decline. Consequently, the major goals of the aging researches are focused on the development of the replacement strategy of the aged organs or cells, based on immortalizing tools, stem cells or artificial substitutes. But recently, a new concept of functional recovery has been introduced on the basis of the functional restoration of the responsiveness of the senescent cells toward a variety of agonists, including growth factors.

Keywords: caveolin, aging, gate keeper, restore, replace

Prevention of T cell ageing will rejuvenate anti-cancer efficacy

Authors: G. Pawelec
Audio: (Audio) (Slides)

Tumour cells are at least initially immunogenic and can be recognised and destroyed by T lymphocytes. Tumours escape this immune destruction by a wide variety of strategies including T cell suppression. Many of the characteristics of the dysfunctional T cells found associated with tumours are shared with those found in ageing.

Keywords: cancer, immunotherapy, tumour escape, chronic antigenic stress, T cell cultures

Targeting Age-Related Modification and Dysfunction of Heart Cell Membranes: Engineering Reversal with Omega 3 Fatty Acids and Antioxidants

Authors: S. Pepe, S.J. Sollott, F.L. Rosenfeldt, E.G. Lakatta
Audio: (Audio) (Slides)

A key feature of the heart in advanced age is a reduced threshold for tolerance to Ca2+ loading during events that stimulate increased Ca2+ entry, such as augmented cardiac work, oxidative stress or post-ischemic reflow. Age-associated modification of cardiac membrane composition is a major factor underlying the relative Ca2+ intolerance and reduced capacity for invoking intrinsic cardioprotective mechanisms that expose a greater vulnerability to ischemic injury.

Keywords: omega-3 PUFA, cardiolipin, signal transduction, mitochondria, antioxidants

Mechanism(s) responsible for exercise-induced cardioprotection

Authors: S.K. Powers
Audio: (Audio)

Myocardial ischemia-reperfusion (I-R) injury is the major event contributing to the morbidity and mortality associated with coronary artery disease. Although several factors contribute to I-R-mediated myocardial injury, compelling evidence indicates that generation of radicals and other reactive oxygen species are important mediators of this type of cardiac damage. Importantly, endurance exercise has been shown to provide significant cardioprotection against an I-R insult. This tutorial lecture will discuss the mechanism(s) responsible for exercise-mediated cardioprotection.

Keywords: , , , ,

Possibility increase life span of animals and man more than 1.5-2 times

Authors: L.Yu. Prokhorov

Purpose: The possibility of 1.5-2 times increase of animals life span (LS) by reduction of cell proliferation rate and a metabolism had been shown earlier on fish, amphibians, reptiles, birds, and mammals (Prokhorov, 1999, 2003). The purpose of the present work to analyze an opportunity to increase LS the men and animals more than in 1.5-2 times.

Keywords: Life span, Cell culture, Metabolism, Telomerase,

Age-related impairment of the transcriptional responses to oxidative stress in the mouse heart

Authors: M. Edwards, D. Sarkar, R. Klopp, J.D. Morrow, R. Weindruch, T.A. Prolla
Audio: (Audio) (Slides)

To investigate the transcriptional response to oxidative stress in the heart and how it changes with age, we examined the cardiac gene expression profiles of young (5-mo-old), middle-aged (15-mo-old), and old (25-mo-old) C57BL/6 mice treated with a single intraperitoneal injection of paraquat (50 mg/kg). Mice were killed at 0, 1, 3, 5, and 7 h after paraquat treatment, and the gene expression profile was obtained with high-density oligonucleotide microarrays.

Keywords: aging, microarray, oxidative stress, ,

An epidemiology study

Authors: M.A. Qajar Koohestani, B. Shabankhani

Introduction: Coexistence between man and livestock has a very long history. This contact had led to the cause of more than 150 disease common between man and animals. Of such disease, plaque, anthrax. brucellosis and mad cow disease are highly life threatening and cause severe epidemies. considering the climatic conditions of mazandaran province, a research as designed and performed in order to delermin the hyginic conditions of cattle dairy farms during 2001-2002.

Keywords: dairy farms, cattle, Environment health, ,

Molecular mechanisms of anti-ageing hormetic effects of repeated mild heat stress on human cells

Authors: S.I.S. Rattan
Audio: (Audio) (Slides)

In a series of experimental studies we have shown that repetitive mild heat stress has anti-ageing hormetic effects on growth and various other cellular and biochemical characteristics of human skin fibroblasts undergoing ageing in vitro.

Keywords: hormesis, cellular aging, kinases, heat shock,

Selenium, glutatione peroxidase, glutathione and malondialdehyde levels in elderly subjects from the Belfast Elderly Longitudinal Free-living Aging Study (BELFAST)

Authors: I.M. Rea, D. McMaster, J. Donnelly, L.T. McGrath, I.S. Young

Background: The free radical theory of ageing suggests that life span is limited by the ability of the organism to cope with damaging free radical reactions and it could be expected that the activity of antioxidant enzymes should decline with increasing age. The seleno-dependent enzyme gluthathione peroxidase (GSHPx) is important in antioxidant systems by its inactivation of hydrogen peroxide while sulphur-containing amino acids maintain glutathione levels to support the glutathione peroxidase reaction.

Keywords: , , , ,

Molecular Mechanisms and Signaling Pathways in Muscle Atrophy in Immobilization and Aging

Authors: A.Z. Reznick, M. Bar-Shai
Audio: (Audio) (Slides)

Aging humans and other mammals lose a substantial amount of their skeletal muscle mass in advanced age, a process termed "Sarcopenia of Old Age". In recent years, mounting evidence has suggested that both extracellular and intracellular degradation processes take place as a result of muscle injury or limb immobilization. These include degradation of extracellular matrix proteins by matrix metalloproteinases (MMPs), and degradation of intracellular proteins by the ubiquitin- proteasome system , lysosomal and calcium- dependent proteases.

Keywords: NF-kB, proteolysis, oxidative stress, aging ,

Regenerative medicine: Antagonic-Stress® therapy in stress and aging. I. Preclinical synthesis - 2003

Authors: D. Riga, S. Riga, F. Schneider

Antagonic-Stress® (AS) drug represents an original synergistic biological composition, with new cell-trophic-regenerative and antioxidative-neurometabolic-cerebrovascular actions (1). AS formula was successively developed for the etio-pathogenic and concomitant interventions in stress, wear and tear, as well as in aging processes (2,3).

For this reason, AS drug was tested in multiple comparative experimental patterns (laboratory animals: rats, mice and guinea pigs):

Keywords: regenerative medicine, anti-stress and anti-aging drug, preclinical studies, synthesis ,

Prolongevity medicine: Antagonic-Stress® drug in stress, aging and related diseases. II. Clinical review - 2003

Authors: S. Riga, D. Riga, F. Schneider

Antagonic-Stress® (AS) drug represents an original concept and a therapeutical strategy in the management of stress, aging and related diseases (1). AS homeostatic-regenerative actions have been confirmed by multiple and comparative, independent and AS authors' researches in preclinical - animal studies (previous paper in this congress) and also in multi-center clinical - human trials (2,3).

Keywords: prolongevity medicine, anti-stress and anti-aging drug, clinical studies, review ,

Quest for Mitochondrial Markers of Impaired Post-Ischaemic Functional Recovery in the Ageing Heart and for Clinical Therapeutic Strategies

Authors: F.L. Rosenfeldt, S. Pepe, A. Hadj, F. Miller, P. Nagley
Audio: (Audio) (Slides)

A hallmark of ageing is the diminished ability to recover from stress. Cardiac surgery imposes a major oxidative stress on the heart that is predictable in magnitude and timing. Although many elderly patients respond well to this stress, others of the same chronological age do not. Our aims were: 1. To identify molecular markers of biological age that would predict the response to surgical stress. 2. To devise therapeutic strategies to improve the post-surgical response of the heart.

Keywords: heart, ageing, mitochondria , ,

Prometheus' vulture and the promise of stem cells

Authors: N. Rosenthal, A. Musaro
Audio: (Audio) (Slides)

When Prometheus transgressed the will of the ancient gods to steal fire for mankind, Jupiter had the great Titan chained to the side of Mount Caucasus where a vulture preyed daily on his liver, which was renewed as quickly as it was devoured. We mere mortals do not possess so vigorous a regenerative capacity, but the legend captures well the remarkable potential of certain mammalian tissues to rebuild themselves. Tissue-restricted regeneration might explain why some organs, lacking a sufficiently robust progenitor cell population, do not regenerate as well as others.

Keywords: Stem cells, muscle, aging, injury, regeneration

Serum heat shock protein 70 and the 2437T allele of the heat shock protein 70-Hom gene in the BELFAST study

Authors: O.A. Ross, M.D. Curran, K.A. Crum, Y.A. Barnett, D. Middleton, I.M. Rea

Introduction:Heat shock protein Hsp70 has been implicated in the ageing process and in diseases accompanying increasing.

Methods: We have measured heat shock protein 70 and anti-heat shock protein 70 in serum in subjects from the Belfast Elderly Longitudinal Free-living Aging Study (BELFAST) together with the frequency of the functional polymorphism, T2437C transversion (MetThr), in the HSP 70-Hom gene using oligonucleotide probes. Hsp70 declined significantly with age (p=0.03). Anti-heat shock 70 antibody showed a modest increase with age (p=0.02)

Keywords: , , , ,