Abstract Archive

This searchable list includes the abstracts of all presentations given at a conference organised as part of the SENS series. We regret that the videos recorded at SENS3 and SENS4 are currently unavailable.

Designing enzymes ab initio

Authors: K.N. Houk, G. Kiss, F. Schoenebeck, S.A. Johnson, S. Kim, G. Nosrati

The computational design of enzymes for new reactions, and the expression of these designed proteins and measurement of catalytic activity have been carried out in collaboration with David Baker at the University of Washington and Stephen Mayo at Caltech. This protocol holds promise for the production of new therapeutic enzymes. Catalytic sites are designed through quantum mechanical calculations, and these theoretical catalytic sites ("theozymes") are incorporated into stable protein scaffolds with the Rosetta programs of Baker et al.

Keywords: catalysis, protein, quantum mechanics, molecular dynamics,

Reprogramming Somatic Cells to Pluripotency Using Small Molecules

Authors: J. Ichida

Organiser's Note: The presenter of this talk withheld their permission for video to be published.

Keywords: , , , ,

Antiviral agents inhibit Alzheimer's disease-like changes in herpes simplex virus-infected cells in culture

Authors: R.F. Itzhaki, A.L. Frost, M.A. Wozniak

The causes of Alzheimer's disease (AD) in non-familial cases, which comprise the vast majority, are unknown. We have implicated herpes simplex virus type 1 (HSV1) as a major factor, on discovering that it resides latently in many elderly brains (J. Med. Virol., 1991, et seq.), that in carriers of the type 4 allele of the apolipoprotein E gene (APOE-ε4) it confers a strong risk of AD (Lancet, 1997), and that it reactivates in brain, possibly recurrently (J. Med. Virol., 2005).

Keywords: Alzheimer's disease, herpes simplex virus type 1, antivirals, amyloid, tau

Quenching the "Fires" of Inflammatory and Oxidative Stress: Implications for Healthy Brain Aging

Authors: J. Joseph, B. Shukitt-Hale, D. Fisher, L. Willis

Organiser's Note: The presenter of this talk withheld their permission for video to be published.

Keywords: Inflammation, Oxidative stress, Brain aging, ,

Telomeres and Reproductive Aging

Authors: D.L. Keefe, L. Liu

Infertility, miscarriage and aneuploid offspring increase with age in women, and meiotic dysfunction underlies reproductive aging. How aging disrupts meiotic function in women remains unclear, but as women increasingly delay attempts at childbearing, solving this problem becomes an urgent priority. Telomeres, which consist of a (TTAGGG)n repeated sequence and associated proteins at chromosome ends, mediate aging in mitotic cells, and also may mediate the effects of aging on meiosis. Telomeres shorten both during DNA replication and from the response to oxidative DNA damage.

Keywords: telomeres, aging, meiosis, reproduction, recombination

Ultra Fast DNA Sequencing by Transmission Electron Microscopy

Authors: W. Andregg, M. Andregg, K. Kemmish, J. Hall, M. Hamalainen

Halcyon Molecular has developed an advanced DNA manipulation technology that enables ultra-low-cost and ultra-fast sequencing by transmission electron microscopy (TEM). We've termed this method IMPRNT for Individual Molecule Placement Rapid Nano Transfer. Fundamental physical differences between TEM and fluorescence based methods allow us to leapfrog other approaches by orders of magnitude on speed, cost and quality. We have recently demonstrated the proof-of-principle for using IMPRNT to sequence DNA.

Keywords: DNA Sequencing, Electron Microscopy, Nanotechnology, ,

Controlling reactive oxygen species in skin at their source to reduce skin aging (ageLOK Technology)

Authors: D.G. Kern, Z.D. Draelos, D.J. Morré, D.M. Morré

Previous work comparing dermis and epidermis from either sun-protected or sun-exposed skin areas plus sera from female subjects ages 28 to 72 years were analyzed for arNOX activity which correlated with oxidative skin damage. ArNOX is a cell surface-located hydroquinone oxidase capable of superoxide generation. The arNOX activity is shed into body fluids and may be monitored in sera, or saliva with equivalent results. In these experiments superoxide production was measured spectrophotometrically using reduction of ferricytochrome c as the quantitation method.

Keywords: Age-related ENOX, Cell-surface oxidase, Reactive oxygen, Skin aging, Advanced Glycation Endproducts

Influence of food signals on ageing and survival

Authors: A.V. Khalyavkin, V.N. Krutko

Life expectancy and health depend not only on hereditary potential, in utero conditions, accidents, environmental features and style of life, but also from quality and quantity of a food. Caloric restriction is one of the most infallible methods for life extension. Hypo caloric animals reveal biological age much younger than the biological age of ad libitum control.

Keywords: food signalling, plasticity of ageing, control systems, external influences on ageing,

The updated view concerning possibility to grow old without senescence

Authors: A.V. Khalyavkin

Many times ago Bernard Strehler prophetically said: "There is no inherent property of cells or of metazoan organization which by itself precludes their organization into perpetually functioning and self-replenishing individuals". It is clear today that the normal somatic cell lines are potentially immortal like cancer cells and germ-line cells.

Keywords: external influences on ageing, non-senescence, rejuvenation, ,

Regression Analysis of Walking Parameters for the Age Predictive Equation

Authors: K. Kikkawa, H. Okada, T. Mori

When we estimate somebody's chronological age, we use their facial features, or the way they move as the basis of the estimate. In this sense, we are solving the inverse problem adopting the statistical thinking of regression analysis with the kinematic variables as the explanatory or independent variables, and age as the dependent variable.

Keywords: kinematic and kinetic parameters, walking data base, Japanese, regression analysis,

Designing Enzymes Ab Initio - Kemp-Eliminases, Cysteine-Esterases, and Diels-Alderases

Authors: G. Kiss, K.N. Houk

Nature's proficient catalysts are responsible for a great variety of difficult chemical transformations that occur under mild conditions with remarkable efficiency and unmatched specificity. The design of novel enzymes has the potential of making these features accessible to synthetic organic catalysis, which constitutes a considerable practical value to biotechnology, pharmaceutical synthesis, and industrial processes.

Keywords: design, therapeutic enzymes, QM, MD,

Crack avoidance during cryopreservation attempts

Authors: K. Kletetschka, G. Kletetschka

This investigation tests whether fracture formation in cryo-preserved ice containing bodies could be affected by alternating magnetic field. The experiment was done on ion containing water inside a plastic container. Magnetism was used to stabilize/balance the pressure exerted during the freezing process. First we tested whether steady application of strong magnetic field has any positive effect on fracture formation, namely, whether a continuous magnetic field exposure can induce any specific fracture pattern.

Keywords: cryopreservation, magnetism, eddy currents, fractures, alternating magnetic field

Long-Term Dietary Supplementation with a Yang-Invigorating Chinese Herbal Formula Increases Lifespan and Mitigates Age-Associated Declines in Mitochondrial Antioxidant Status and Functional Ability of Various Tissues in Male and Female C57BL/6J Mice

Authors: K.M. Ko, P.Y. Chiu, H.Y. Leung, A. H.L. Siu, N. Chen, M.K.T. Poon

Previous work in our laboratory has shown that long-term treatment with Vigconic 28 (VI-28), a Yang-invigorating herbal formula used for the promotion of overall wellness in Chinese medicine, can enhance both mitochondrial antioxidant status and functional ability, and protects against oxidant injury of various rat tissues. To investigate whether VI-28 could affect survival of aging animals, male and female C57BL/6J mice were given a VI-28 supplemented diet (0.05 and 0.5%, w/w) starting at 36 weeks of age, until death.

Keywords: Longevity, Mitochondrial decay, Antioxidants, Chinese herbs,

Manipulation and Derivation of the Hematopoitic Stem Cell Niche

Authors: D. Kraft, A. Czechowicz, C.K. Chan, C.C. Chen, D. Bhattacharya, I.L. Weissman

Introduction: Identification and understanding of the cells and processes that can generate, sustain and influence the hematopoieitic stem cell (HSC) niche and hematopoiesis are critical for the development of a more comprehensive knowledge of normal hematopoiesis, stem cell homing, trafficking, differentiation, hematopoietic pathology and more effective and less toxic clinical interventions. Growth and renewal in many tissues are initiated by stem cells, supported by the microenvironment (niche) in which they reside.

Keywords: stem cell, niche, transplantation, bone marrow, hematopoietic stem cell

Practice of Postponing Human Aging

Authors: Ü. Kristjuhan

Let us define aging as the accumulation of damage in the human organism during lifetime, organismal aging. So there is no borderline between aging and age-related diseases; "accumulation of damage" includes also these diseases. Average life expectancy increases in most developed countries by 0.15-0.2 years every year. It is already 83 years in Japan. Expected lifetime in good health is increasing approximately as much as the overall life expectancy. Consequently, at present aging processes in human organisms are postponing.

Keywords: postponing aging, health, humans, efficiency,

Postponed Aging in University Teachers

Authors: Ü. Kristjuhan, E. Taidre

Organiser's Note: The presenter of this talk withheld their permission for video to be published.

Among professional groups, professionals have average later onset of age-related diseases and several years longer life expectancy. Probably the cause is the good complex of health determinants including fewer risk factors. However, the professional's life expectancy exceeds usually not more than 10% the average life expectancy of unskilled workers.

Keywords: postponing aging, prolonging life, university teachers, ,

Identification of pro-ageing molecular mechanisms in the thymus

Authors: K. Kvell, Z. Varecza, G. Miskei, S. Parnell, G. Anderson, E.J. Jenkinson, J. Pongracz

The increase of the elderly population in developed societies imposes considerable healthcare and economic burdens. To alleviate these problems, research must be devoted to the identification of molecular mechanisms underlying senescence. As the thymus undergoes significant histological changes during ageing, the thymus is an organ that provides an excellent model system to study senescence. Also, age-associated thymic involution has clear physiological impacts by inhibiting de novo T-cell selection followed by impaired immune responses.

Keywords: thymus, involution, adipose tissue, ,

Applied Healthspan Engineering

Authors: J.W. Larrick, A. Mendelsohn

We define Applied Healthspan Engineering as the use of behavioral, pharmacological, biomechanical, and regenerative means to maximize wellness with increasing chronological age and eventually overcome aging. Significant progress has been made in understanding the fundamental cell physiology of aging and age-related diseases. To catalyze discussion, we survey therapeutic approaches supported by current knowledge and chart a roadmap of those that in the near-term may be developed to extend the "healthspan".

Keywords: , , , ,

Strikingly opposite correlations of nuclear and mitochondrial DNA base composition with maximum life span in mammals

Authors: G. Lehmann, V.E. Fraifeld, A. Zagorodniy, K.K. Muradian

DNA base composition is a concise quantitative index highlighting the counterbalance of mutagenic and repair factors which shape the genome stability and eventually aging patterns. As it has recently been shown by us and others, mitochondrial DNA (mtDNA) base composition exhibits unusually strong correlations with maximum life span (MLS). Little efforts, however, have been invested to clarify if there are any analogous links for nuclear DNA (nDNA).

Keywords: longevity, mammals, nuclear and mitochondrial DNA, ,

Amyloid formation: age-related mechanism in Creutzfeldt-Jakob disease?

Authors: L. Luers, G. Panza, C. Dumpitak, F. Henke, T. Aygenim, D. Willbold, E. Birkmann

Amyloid protein aggregates play a major role in many age-related diseases (Yankner et al., 2008) like Creutzfeldt-Jakob disease (CJD), Alzheimer’s disease, or Parkinson’s disease. Characteristically for these diseases is the aggregation and deposition of certain naturally occurring proteins in the central nervous system. Prion protein (PrP) aggregates can be found in the brain of CJD patients, in case of AD aggregates of amyloid-beta and in PD aggregates of α-synuclein occur.

Keywords: CJD, amyloid, polymorphism, prion-protein, fibrillisation

Non-invasive neonatal thymus graft into the axillaries lodge extend lifespan of mice

Authors: A. Basso, M. Malavolta, F. Piacenza, F. Marcellini, R. Papa, E. Mocchegiani

Among the organs which may act as clocks for aging, particular interest is for thymus due to its precocious and progressive involution and because it controls important functions for survival, such as the immunological ones. But thymus is also important for an endocrine role. Neonatal thymus graft exerts rejuvenating action on non immunological various functions found altered in old mice. These results are obtained with thymus grafted under kidney capsule in invasive manner, and maybe this is why precedent survival kinetics doesn?t show any changes on median and maximal life span.

Keywords: thymus graft, axillaries lodge, survival, rejuvenation strategies,

A novel bioengineering clinical device testing cellular homeostatic potentials to customize and monitor nutritional-related age-management interventional strategies

Authors: G. Mariani, F. Dellaglio, F. Marotta

There is an ever increasing evidence linking body composition abnormalities with a biological cascade of pro-inflammatory cellular events eventually leading, mostly causatively but also epiphenomenically, to the onset of a large part of chronic degenerative diseases. However, most clinically-applicable devices assessing body composition harbour a number of drawbacks and hardly assess in a objectively manner the phenomena taking place at a cellular membrane level.

Keywords: Potential Homeostatic Structure Analysis, Rilevo technology, body composition, cellular potential, nutraceutical intervention

Progression of atherosclerotic lesion in the arteries and related gene expression: protective effect from a phytotherapeutic agent

Authors: F. Marotta, M. Harada, E. Minelli, H. Yadav, A. Lorenzetti, N. Locorotondo

The inflammatory nature of atherosclerosis has prompted efforts to prevent development and/or progression of disease by targeting inflammatory mediators, including cytokines, chemokines, and MMPs. Indeed, endothelial cell dysfunction in eNOS expression by TNF-α is involved in the pathogenesis of vascular diseases with related pathologic changes such as increased expression of adhesion ICAM-1, VCAM-1 and E-selectin. Moreover, oxidative modification of LDL is thought to play a key role in the pathogenesis of atherosclerosis.

Keywords: TNF-alpha, LD-oxidation, atherosclerosis, DTS,

Regulating redox balance gene expression in healthy individuals by nutraceuticals: a pilot study

Authors: F. Marotta, K. Koike, A. Lorenzetti, S. Jain, Y. Metugriachuk, P. Mantello, N. Locorotondo

One of the most important emerging areas of nutrition science is represented by the area of nutrigenomics, i.e. the effect of diet on gene expression and chromosomal structure, and the extent to which genetic differences between individuals affect response to a specific dietary intake, functional food or supplement in terms of a defined health outcome. While there is wide debate on which extent oxidative stress is a causative or epiphenomenic event in aging and in chronic diseases, the question arises as to whether supplemental antioxidants are beneficial.

Keywords: redox balance, gene expression, fermented papaya preparation, ,

The Lifenaut Project: a multifaceted experiment in data storage for future machine consciousness learning

Authors: N. Mayer

Consciousness as a formal field of study has witnessed rapid growth in the areas of psychological, philosophical, biological, and computational research in recent years. In particular there has been a bloom of published work related to the replication of humanlike consciousness via hardware and software applications (Aleksander, Haikonen, Holland, Kurzweil and many others) culminating with the release of the new Internationational Journal of Machine Consciousness just a few days before this writing.

Keywords: , , , ,

Genetic Variation and the Pathways to Successful Ageing

Authors: D. Melzer

Core to successful aging is delaying the onset of age-related disease (ARD) until late in life. Several hundred genome wide studies identifying associations between ARDs and over 85% of common genetic variation across the human genome have now been published. Most are large-scale, with robust identification and replication of findings.

In reviewing the results, I will address two basic questions:

Keywords: genetic, polymorphisms, successful ageing, disease, cell senescence

Accumulated Damage is not the Root Cause of Aging

Authors: J. Mitteldorf

The idea that bodies wear out with age is so ancient, so pervasive, and so deeply rooted that it affects our thought in unconscious ways. Indeed, many aspects of aging, e.g. oxidative damage and protein cross-linkage are characterized by increased entropy in biomolecules. However, it has been a scientific consensus for more than a century that there is no physical necessity for such damage. Living systems are defined by their capacity to gather order from their environment, concentrate it, and shed entropy with their waste.

Keywords: , , , ,

The SENS Foundation Academic Initiative (SENSFAI)

Authors: K. Moody

The study of human aging requires an implementation of translational approaches which many students are simply not exposed to in their general education. Current educational paradigms present an opportunity to easily and inexpensively train students in these methods and cultivate interest in aging research.

Keywords: , , , ,

Molecular Nanotechnology in the Real World: How Feasible is a Nanofactory?

Authors: P. Moriarty

Nanotechnology, and in particular the molecular manufacturing and nanoassembler concepts first put forward by K Eric Drexler [1] in the eighties, have been lauded as key enabling technologies to advance the human condition. At the core of this approach is the manipulation of single atoms and molecules using computer-controlled actuators or probes, which he argues will enable the assembly of "virtually anything" from basic raw materials [2].

Keywords: molecular nanotechnology, mechanosynthesis, diamondoid, ,

ArNOX-- An active generator of reactive oxygen species in the skin and sera of aging individuals subject to external modulation

Authors: D.M. Morré, C. Meadows, D.J. Morré

Organiser's Note: The presenter of this talk withheld their permission for video to be published.

Keywords: Age-related ENOX, Cell-surface oxidase, Reactive oxygen, Atherogenic risk, oral supplements

Aging related NADH oxidase (arNOX) response to dietary supplementation. The French Paradox revisited

Authors: D.J. Morré, D.M. Morré, T.B. Shelton

Aging related cell surface oxidase (arNOX) specific activities (increasing between age 30 and age 50 to 65) correlate with a number of aging-related disorders including LDL oxidation as a precondition to atherosclerosis and oxidation of collagen and elastin as a major contributor to skin aging. Because of the postulated importance of arNOX to these and possibly other aging related disorders, NOX Technologies Inc.

Keywords: Age-related ENOX, Cell-surface oxidase, Reactive oxygen, Atherogenic risk, French Paradox

Pharmacological inhibition of phosphoinositide 3- and TOR-kinase improves survival of Drosophila melanogaster

Authors: A.A. Moskalev, M.V. Shaposhnikov

on the search and study of chemical compounds and drugs, possessing lifespan extension for model animals. Recent progress in our understanding of genetic mechanisms of aging and longevity gives an opportunity to select some enzymes as targets for pharmacological correction. The phosphoinositide 3-kinase (PI3K) and TOR-kinase cascades are affected in some long-lived mutants of different animals, such as nematodes and mice. The purpose of this study is to investigate the geroprotector efficiency of the inhibitors of enzymes that are known to be affected in long-lived mutants.

Keywords: Drosophila, longevity, LY-294002, wortmannin, rapamycin

"ORION" - a glimpse of hope in life span extension?

Authors: K.K. Muradian, V.N Bondar, V.V. Bezrukov, O.I. Zhukovsky, V.I Polyakov, N.A. Utko

Effects of a multicomponent drug Orion on life span and stress-resistance of D.melanogaster were studied. Orion doubled flies survival at stresses primarily associated with micromolecule denaturation. However, no positive effects were found when modeling oxidative stress.

Keywords: lifespan extension, stress-resistance, Orion, drosophila,

NUMT ("new mighty") hypothesis of longevity

Authors: K.K. Muradian and V.E. Fraifeld

Over 99% of protobacterial genes "immigrated" into the safer vicinity of nuclear DNA (nDNA) after the endosymbiosis and establishment of "peaceful" coexistence of mitochondrial DNA (mtDNA) and nDNA within the eukaryotic cell. It seems, however, that aberrant attempts to implement the remaining few mitochondrial genes into nDNA in the form of mtDNA segments of various size, known as NUMT (from: nuclear mitochondrial, pronounced "new mighty"), have never been ceased. The relevance of NUMT to aging and longevity remains completely unknown.

Keywords: DNA, longevity, mitochondria, nucleus, NUMT

Exercise intensity and antioxidant ability

Authors: J. Nagasawa, K. Kikkawa, T. Takai, A. Sakaguchi, H. Ohno

The reactive oxygen species cause tissue damage. Because aerobic metabolism increases during a physical exercise, it is very likely that the productions of the active oxygen increases exercise intensity dependently. So, the intense exercise may result in damage to a cell membrane or a nucleic acid.

Keywords: oxidative stress, exercise intensity, ESR, ROS,

How Common and Clinically Relevant is Localised Cardiac Amyloidosis in Older Patients with Heart Failure and Preserved Left Ventricular Systolic Function (Diastolic Heart Failure)?

Authors: N.P. Nikitin, J.G. Cleland

Cardiac amyloidosis as part of systemic amyloidosis is considered a rare condition. However, there is growing evidence (acquired predominantly in necropsy studies) that isolated cardiac amyloidosis of various biochemical identities is much more common in older population and could contribute to the development of heart failure and atrial fibrillation.

Keywords: amyloidosis, heart failure, magnetic resonance imaging, ageing,

Why T-cells go out of whack with aging and what to do about it

Authors: J. Nikolich-Zugich

Organiser's Note: The presenter of this talk withheld their permission for video to be published.

Keywords: T-cells, CMV, aging, T-cell receptor, repertoire

How intracellular redox status regulates precursor cell function

Authors: M. Noble

Getting older, getting more oxidized and having reduced abilities for regeneration all are associated in time - but are they associated by mechanism? Cellular biological and physiological analyses suggest they may well be so. Our studies have shown that small (

Keywords: redox, redox/Fyn/c-Cbl pathway, precursor cell function, signaling pathways,

Recent progress in pharmacological amelioration of telomere shortening

Authors: N. Patton

Organiser's Note: The presenter of this talk withheld their permission for video to be published.

Keywords: , , , ,

Beneficial catalytic immunity to amyloid β peptide

Authors: S. Paul, S. Planque, Y. Nishiyama

Adaptive synthesis of antibodies (Abs) to microbes is a powerful defense against infectious disease. If Abs express the catalytic function, their efficacy is enhanced further. We explored the ability of the immune system to produce Abs that catalyze the degradation of amyloid β peptide (Aβ), a self-antigen with a central role in neurodegenerative processes. Polyclonal IgM autoantibodies from the blood and cerebrospinal fluid of humans without dementia hydrolyzed Aβ specifically. The catalytic activity improved as a function of age.

Keywords: catalytic antibodies, amyloid peptide, autoimmunity, ,

An altered hippocampal histone code is associated with age-dependent memory impairment

Authors: S. Peleg, F. Sananbenesi, J. Wittnam, P. Cota, S. Burkhardt, L. Farinelli, A. Fischer

During aging, a marked gradual decline is frequently observed in the performance of cognitive and memory tasks. Importantly, aging is also the major risk factor for neurodegenerative diseases such as Alzheimer's disease (AD), which affect millions of people worldwide. The reasons for the decline in brain function during aging, is however not well understood.

Keywords: chromatin, learning, epigenetic, histone, HDAC

Treatment and prevention of amyloidosis

Authors: M.B. Pepys

Organiser's Note: The presenter of this talk withheld their permission for video to be published.

Keywords: Amyloidosis, Treatment, Serum amyloid P component, Transthyretin,

The Lifestar World Health Initiative - The Imperative And Means For Increasing Global Funding For Aging Research

Authors: K. Perrott, B. Logan, P. Logan, W. Millard

With a cumulative economic burden of 57 trillion dollars over the next 14 years in direct and indirect costs in the United States alone (Milken 2003), the cost and care of the chronic diseases of aging are poised to overwhelm already foundering economies of the world. Indeed the global impact of non-communicable disease is growing and accounts for 44% of the disease burden in developing nations and 85% in the developed world.

Keywords: economics, collaboration, aging, therapies, demographics

Senescence versus rejuvenation of immune system in heterochronic parabiotic partners

Authors: I.N. Pishel, A.E. Rodnichenko, T.A. Dubiley, P.P. Klymenko, D.V. Shytikov, T.N. Orlova, G.M. Butenko

Introduction. Age-related reduction in immune function is well documented. The reduction is explained by many factors, including decreased thymus function, dramatic decrease in the proportion of naive T cells with a concomitant increase in T cells with memory phenotypes, altered cytokine production by APC, and alterations in lymphocyte intracellular signal transduction pathways.

Purpose. To prevent these changes and for old partner rejuvenation, we undertook a study to create heterochronic parabiotic pairs with common blood circulation.

Keywords: , , , ,

Experimental Motivation for Using Cellular Therapy for Alzheimer's Disease Treatment

Authors: R.A. Poltavtseva, V.N. Nesterova, N.I. Medvinskaya, A.N. Samokhin, I.V. Artyukhov, A.G. Peregudov, G.T. Sukhikh , N.V. Bobkova

At present the focus of Alzheimer's disease (AD) studies tends to shift from investigating exclusively the beta-amyloid plaque formation to considering other possible mechanisms contributing to its pathogenesis as well. We used olfactory bulbectomized (OBE) mice as a model for sporadic Alzheimer's disease. Our data show that 3 weeks after the olfactory bulbs removal in mice an AD-like neurodegenerative process develops concurrently with the spatial memory impairment. Simultaneously a maximum in stem cell proliferation is observed.

Keywords: Alzheimer's disease, stem cells, transplantation, mice,

Affordable rejuvenation: a prototype facility in action

Authors: A.F. Prokopov, J. Reinmuth

The majority of regenerative and rejuvenative interventions are still in an early research phase. Meanwhile, the well-proven natural interventions, such as various forms of caloric/nutritive restriction, adaptive and bio-oxidative treatments are not universally embraced because of their low compliance. Yet, the applicability of such interventions is significantly improved by "engineering" them for higher efficiency and better user-friendliness.

Keywords: regenerative treatment, intermittent nutritive-and oxygen restriction, , ,

A case of recovery from dementia, following rejuvenative treatment

Authors: A.F. Prokopov

A female patient, born in 1931, suffered progressive mental decline for about one year. She could no longer conduct usual activities and home chores. She was unable to hear without a hearing aid. Her brain MRI from Febr. 2008 showed massive degenerative changes.

Keywords: dementia recovery, integrative treatment, IHT, intermittent nutritive-and oxygen restriction,

Senescent T cell removal using magnetic antibodies

Authors: J. Rebo, K, Casey, M. Hamalainen, J. Scholendorn

A major type of unwanted cell that accumulates in aging are anergic T cells of the aging immune system. these cells often have virus specific T cell receptors, as well as other surface markers that distinguish them from their youthful counterparts. Here we consider two surface markers thought to define these cells in mice, CD8 and KLRG1, as a means we developed to remove these cells from the blood of C57BL6 mice.

Keywords: immunosens, anergic T cells, CD8, KLRG1,

Cellular repair in the non-human primate brain with human neural stem cells and multiple fetal cells grafts.

Authors: J.R. Sladek Jr., E.Y. Snyder, K.B. Bjugstad, T.J. Collier, B. Blanchard, Y.D. Teng, R.H. Roth, J.D. Elsworth, D.E. Redmond Jr.

We demonstrated previously that grafts of fetal dopaminergic neurons can reverse motor deficits induced by the toxin, MPTP, in parkinsonian, non-human primates as a test of feasibility of cellular repair in a complex nervous system. Clinical trials followed in both young and aged Parkinson patients with encouraging results including patients who showed substantial improvement in mobility as well as reduction in medications for up to 8-10 years.

Keywords: stem cells, Parkinson's disease, cellular repair, tract reconstruction,

Superiority of regeneration in middle-aged mice: the neural-crest connection

Authors: B.P. Reines

For more than a century, the accepted wisdom has been that true regeneration is only possible in either very simple or very young animals. Our recent findings which tend to challenge that framework are: 1) a blastema-based type of regeneration is a generic property of repair in excisional wounds in mouse ears, and 2) middle-aged mice of common strains are better regenerators than young post-weaning mice. However, viewed from a deeper biological perspective, our findings tend to corroborate the accepted view.

Keywords: regeneration, neural crest, age, mammals,