Proving the Principle
I am working on a new experiment with nonspecific peroxidase enzymes and ALE-modified proteins. I am reacting BSA with malondialdehyde (MDA) to modify it with fluorescent crosslinks, which I can measure using ELISA. I will then react common, non-specific enzymes like horseradish peroxidase (HRP) with the modified BSA and measure if any of the crosslink was degraded. If I can show that an enzyme like HRP is able to access ALE modifications on a protein surface, this would show some promise for using enzymes to degrade damaged proteins in vivo.