Proving the Principle

Posted by Rob O'Callahan on August 03, 2010 | Clearing cells of age-related wastes

I am working on a new experiment with nonspecific peroxidase enzymes and ALE-modified proteins.  I am reacting BSA with malondialdehyde (MDA) to modify it with fluorescent crosslinks, which I can measure using ELISA.  I will then react common, non-specific enzymes like horseradish peroxidase (HRP) with the modified BSA and measure if any of the crosslink was degraded.  If I can show that an enzyme like HRP is able to access ALE modifications on a protein surface, this would show some promise for using enzymes to degrade damaged proteins in vivo.